Journal article
Bucillamine Inhibits UVB-Induced MAPK Activation and Apoptosis in Human HaCaT Keratinocytes and SKH-1 Hairless Mouse Skin
Photochemistry and photobiology, Vol.96(4), pp.870-876
07/2020
DOI: 10.1111/php.13228
PMCID: PMC7387142
PMID: 32077107
Abstract
Ultraviolet B (UVB) radiation is known as a culprit in skin carcinogenesis. We have previously reported that bucillamine (N-[2-mercapto-2-methylpropionyl]-L-cysteine), a cysteine derivative with antioxidant and anti-inflammatory capacity, protects against UVB-induced p53 activation and inflammatory responses in mouse skin. Since MAPK signaling pathways regulate p53 expression and activation, here we determined bucillamine effect on UVB-mediated MAPK activation in vitro using human skin keratinocyte cell line HaCaT and in vivo using SKH-1 hairless mouse skin. A single low dose of UVB (30 mJ cm(-2)) resulted in increased JNK/MAPK phosphorylation and caspase-3 cleavage in HaCaT cells. However, JNK activation and casaspe-3 cleavage were inhibited by pretreatment of HaCaT cells with physiological doses of bucillamine (25 and 100 mu m). Consistent with these results, bucillamine pretreatment in mice (20 mg kg(-1)) inhibited JNK/MAPK and ERK/MAPK activation in skin epidermal cells at 6-12 and 24 h, respectively, after UVB exposure. Moreover, bucillamine attenuated UVB-induced Ki-67-positive cells and cleaved caspase-3-positive cells in mouse skin. These findings demonstrate that bucillamine inhibits UVB-induced MAPK signaling, cell proliferation and apoptosis. Together with our previous report, we provide evidence that bucillamine has a photoprotective effect against UV exposure.
Details
- Title: Subtitle
- Bucillamine Inhibits UVB-Induced MAPK Activation and Apoptosis in Human HaCaT Keratinocytes and SKH-1 Hairless Mouse Skin
- Creators
- Adil Anwar - University of Colorado Anschutz Medical CampusHiba Anwar - University of Colorado Anschutz Medical CampusTakeshi Yamauchi - University of Colorado Anschutz Medical CampusRyan Tseng - University of Colorado Anschutz Medical CampusRajesh Agarwal - University of Colorado Anschutz Medical CampusLawrence D. Horwitz - University of Colorado Anschutz Medical CampusZili Zhai - University of Colorado Anschutz Medical CampusMayumi Fujita - University of Colorado Anschutz Medical Campus
- Resource Type
- Journal article
- Publication Details
- Photochemistry and photobiology, Vol.96(4), pp.870-876
- DOI
- 10.1111/php.13228
- PMID
- 32077107
- PMCID
- PMC7387142
- NLM abbreviation
- Photochem Photobiol
- ISSN
- 0031-8655
- eISSN
- 1751-1097
- Publisher
- Wiley
- Number of pages
- 7
- Grant note
- P30CA046934 / University of Colorado Cancer Center Support Grant P30AR057212 / Skin Diseases Research Cores Grant R01 CA197919 / NIH/NCI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Gates Summer Internship Program 5I01BX001228 / Veterans Affairs Merit Review Awards; US Department of Veterans Affairs Cancer League of Colorado
- Language
- English
- Date published
- 07/2020
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656526702771
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