Journal article
Bulk and single-cell gene expression analyses reveal aging human choriocapillaris has pro-inflammatory phenotype
Microvascular research, Vol.131, pp.104031-104031
09/2020
DOI: 10.1016/j.mvr.2020.104031
PMCID: PMC7396301
PMID: 32531351
Abstract
The human choroidal vasculature is subject to age-related structural and gene expression changes implicated in age-related macular degeneration (AMD). In this study, we performed both bulk and single-cell RNA sequencing on infant (n = 4 for bulk experiments, n = 2 for single-cell experiments) and adult (n = 13 for bulk experiments, n = 6 for single-cell experiments) human donors to characterize how choroidal gene expression changes with age. Differential expression analysis revealed that aged choroidal samples were enriched in genes encoding pro-inflammatory transcription factors and leukocyte transendothelial cell migration adhesion proteins. Such genes were observed to be differentially expressed specifically within choroidal endothelial cells at the single-cell level. Immunohistochemistry experiments support transcriptional findings that CD34 is elevated in infant choriocapillaris endothelial cells while ICAM-1 is enriched in adults. These results suggest several potential drivers of the pro-inflammatory vascular phenotype observed with advancing age.
[Display omitted]
•Bulk or single-cell RNA sequencing were performed on 6 infant and 19 adult human donor choroids.•Differentially expressed genes were identified between infant and adult cells in all choroidal cell populations.•Distinct clusters of pericytes and vascular smooth muscle cells were characterized.•Aged human choroid demonstrated increased pro-inflammatory gene expression, particularly in endothelial cells.
Details
- Title: Subtitle
- Bulk and single-cell gene expression analyses reveal aging human choriocapillaris has pro-inflammatory phenotype
- Creators
- Andrew P Voigt - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaS. Scott Whitmore - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaKelly Mulfaul - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaKathleen R Chirco - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaJoseph C Giacalone - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaMiles J Flamme-Wiese - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaAdam Stockman - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaEdwin M Stone - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaBudd A Tucker - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaTodd E Scheetz - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of AmericaRobert F Mullins - Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States of America
- Resource Type
- Journal article
- Publication Details
- Microvascular research, Vol.131, pp.104031-104031
- DOI
- 10.1016/j.mvr.2020.104031
- PMID
- 32531351
- PMCID
- PMC7396301
- NLM abbreviation
- Microvasc Res
- ISSN
- 0026-2862
- eISSN
- 1095-9319
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000002, name: NIH, award: T32 GM007337, EY024605, P30 EY025580; DOI: 10.13039/100001818, name: Research to Prevent Blindness; DOI: 10.13039/100012201, name: Elmer and Sylvia Sramek Charitable Trust; name: Martin Carver Chair in Ocular Cell Biology
- Language
- English
- Date published
- 09/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; The University of Iowa Institute for Vision Research; Iowa Neuroscience Institute; John and Marcia Carver Nonprofit Genetic Testing Laboratory; Ophthalmology and Visual Sciences
- Record Identifier
- 9984070839602771
Metrics
40 Record Views