Bullfrog oil (Rana catesbeiana Shaw) induces apoptosis, in A2058 human melanoma cells by mitochondrial dysfunction triggered by oxidative stress

Lucas Amaral-Machado; Wógenes N Oliveira; Éverton N Alencar; Ana Katarina M Cruz; Hugo Alexandre O Rocha; Kareem Ebeid; Aliasger K Salem; Eryvaldo Sócrates T Egito

doi:10.1016/j.biopha.2019.109103

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Bullfrog oil (Rana catesbeiana Shaw) induces apoptosis, in A2058 human melanoma cells by mitochondrial dysfunction triggered by oxidative stress

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Bullfrog oil (Rana catesbeiana Shaw) induces apoptosis, in A2058 human melanoma cells by mitochondrial dysfunction triggered by oxidative stress

Lucas Amaral-Machado, Wógenes N Oliveira, Éverton N Alencar, Ana Katarina M Cruz, Hugo Alexandre O Rocha, Kareem Ebeid, Aliasger K Salem and Eryvaldo Sócrates T Egito

Biomedicine & pharmacotherapy, Vol.117, pp.109103-109103

09/2019

DOI: 10.1016/j.biopha.2019.109103

PMID: 31203130

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Abstract

[Display omitted] •Bullfrog oil (BO) trigged important cytotoxicity in human melanoma cells, in vitro.•BO increases the intracellular ROS levels in human melanoma cells.•Apoptosis is the death pathway of human melanoma cells treated with BO.•BO, an option on the development of new products intended for anti-melanoma therapy. Bullfrog oil, an animal oil extracted from the adipose tissue of Rana catesbeiana Shaw, showed promising cytotoxic activity against melanoma cells and, therefore, has the potential to become a pharmaceutical active compound. However, there is a lack of information regarding the pathways involved in its pharmacological activity. Thus, the aim of this study was to investigate and elucidate the cytotoxic effect of this oil against A2058 human melanoma cells. The cytotoxic potential was evaluated by the MTT assay, the cell cycle analysis and the cell death assay. In addition, the apoptotic potential was investigated by (i) the DNA fragmentation using propidium iodide staining analysis, (ii) the evaluation of mitochondrial membrane potential and (iii) the determination of intracellular Reactive Oxygen Species (ROS) level. The results showed that the bullfrog oil was able to promote a time-dependent cytotoxic effect, decreasing cell viability to 38% after 72 h of treatment without affecting the cell cycle. Additionally, the bullfrog oil induced the apoptosis in A2058 cells, increasing up to 50 ± 13% of the intracellular ROS level, maintaining the DNA integrity and promoting an approximate decrease of 35 ± 5% in the mitochondrial membrane potential. It can be concluded that the in vitro cytotoxic effect of the bullfrog oil in A2058 human melanoma cells is mediated by oxidative stress that induces mitochondrial dysfunction, triggering the apoptosis. These unprecedented results highlight the pharmacological potential of bullfrog oil and provide important information to support studies on the development of new pharmaceutical products for complementary and alternative treatments for melanoma.

Apoptosis

Bullfrog oil

Cytotoxicity

Melanoma

Natural products

Details

Title: Subtitle: Bullfrog oil (Rana catesbeiana Shaw) induces apoptosis, in A2058 human melanoma cells by mitochondrial dysfunction triggered by oxidative stress
Creators: Lucas Amaral-Machado - Graduation Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil
Wógenes N Oliveira - Graduation Program in Pharmaceutical Sciences, UFRN, Natal, Brazil
Éverton N Alencar - Graduation Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil
Ana Katarina M Cruz - Laboratory of Natural Polymers Biotechnology (BioPol), UFRN, Natal, Brazil
Hugo Alexandre O Rocha - Laboratory of Natural Polymers Biotechnology (BioPol), UFRN, Natal, Brazil
Kareem Ebeid - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USA
Aliasger K Salem - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USA
Eryvaldo Sócrates T Egito - Graduation Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil
Resource Type: Journal article
Publication Details: Biomedicine & pharmacotherapy, Vol.117, pp.109103-109103
DOI: 10.1016/j.biopha.2019.109103
PMID: 31203130
NLM abbreviation: Biomed Pharmacother
ISSN: 0753-3322
eISSN: 1950-6007
Publisher: Elsevier Masson SAS
Grant note: DOI: 10.13039/501100002322, name: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil; DOI: 10.13039/501100003593, name: Conselho Nacional de Desenvolvimento Científico e Tecnológico
Language: English
Date published: 09/2019
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9984216687102771

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