C. elegans HLH-2/E/Daughterless controls key regulatory cells during gonadogenesis

Michael A Chesney; Ngan Lam; Dyan E Morgan; Bryan T Phillips; Judith Kimble

doi:10.1016/j.ydbio.2009.04.015

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C. elegans HLH-2/E/Daughterless controls key regulatory cells during gonadogenesis

Journal article

Open access

Peer reviewed

C. elegans HLH-2/E/Daughterless controls key regulatory cells during gonadogenesis

Michael A Chesney, Ngan Lam, Dyan E Morgan, Bryan T Phillips and Judith Kimble

Developmental biology, Vol.331(1), pp.14-25

07/01/2009

DOI: 10.1016/j.ydbio.2009.04.015

PMCID: PMC2776051

PMID: 19376107

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Abstract

The Caenorhabditis elegans distal tip cell (DTC) provides a niche for germline stem cells in both hermaphrodites and males. The hermaphrodite distal tip cell (hDTC) also provides “leader” function to control gonadal elongation and shape, while in males, leader function is allocated to the linker cell (LC). Therefore, the male distal tip cell (mDTC) serves as a niche but not as a leader. The C. elegans homolog of E/Daughterless, HLH-2, was previously implicated in hDTC specification. Here we report that HLH-2 is also critical for hDTC maintenance, hDTC niche function and hDTC expression of a lag-2/DSL ligand reporter. We also find that HLH-2 functions in males to direct linker cell specification and to promote both mDTC maintenance and the mDTC niche function. We conclude that HLH-2 functions in both sexes to promote leader cell specification and DTC niche function.

Gonadogenesis

HLH-2, lag-2, Distal tip cell

Leader function

Morphogenesis

Organogenesis

Stem cell niche

Details

Title: Subtitle: C. elegans HLH-2/E/Daughterless controls key regulatory cells during gonadogenesis
Creators: Michael A Chesney - Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1544, USA
Ngan Lam - Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1544, USA
Dyan E Morgan - Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706, USA
Bryan T Phillips - Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1544, USA
Judith Kimble - Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1544, USA
Resource Type: Journal article
Publication Details: Developmental biology, Vol.331(1), pp.14-25
DOI: 10.1016/j.ydbio.2009.04.015
PMID: 19376107
PMCID: PMC2776051
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Publisher: Elsevier Inc
Language: English
Date published: 07/01/2009
Academic Unit: Biology
Record Identifier: 9984217537102771

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