CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

Alice Nomura; Sulagna Banerjee; Rohit Chugh; Vikas Dudeja; Masato Yamamoto; Selwyn M Vickers; Ashok K Saluja

doi:10.18632/oncotarget.3228

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CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

Journal article

Open access

CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer

Alice Nomura, Sulagna Banerjee, Rohit Chugh, Vikas Dudeja, Masato Yamamoto, Selwyn M Vickers and Ashok K Saluja

Oncotarget, Vol.6(10), pp.8313-8322

04/10/2015

DOI: 10.18632/oncotarget.3228

PMID: 25829252

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https://doi.org/10.18632/oncotarget.3228View

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Abstract

CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced "stemness" properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

AC133 Antigen

Animals

Antigens, CD - biosynthesis

Cell Line, Tumor

Epithelial-Mesenchymal Transition - physiology

Female

Glycoproteins - biosynthesis

Heterografts

Humans

Mice

Mice, Nude

Neoplasm Metastasis

Neoplastic Stem Cells - metabolism

Neoplastic Stem Cells - pathology

NF-kappa B - metabolism

Pancreatic Neoplasms - genetics

Pancreatic Neoplasms - metabolism

Pancreatic Neoplasms - pathology

Peptides

Signal Transduction

Details

Title: Subtitle: CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer
Creators: Alice Nomura - University of Minnesota
Sulagna Banerjee - University of Minnesota
Rohit Chugh - University of Minnesota
Vikas Dudeja - University of Minnesota
Masato Yamamoto - University of Minnesota
Selwyn M Vickers - University of Minnesota
Ashok K Saluja - University of Minnesota
Resource Type: Journal article
Publication Details: Oncotarget, Vol.6(10), pp.8313-8322
DOI: 10.18632/oncotarget.3228
PMID: 25829252
NLM abbreviation: Oncotarget
ISSN: 1949-2553
eISSN: 1949-2553
Grant note: T32 DA007097-32 / NIDA NIH HHS R01 CA184274 / NCI NIH HHS R01 CA170946 / NCI NIH HHS R01-CA184274 / NCI NIH HHS R01-CA170946 / NCI NIH HHS R01 CA124723 / NCI NIH HHS T32 DA007097 / NIDA NIH HHS
Language: English
Date published: 04/10/2015
Academic Unit: Anatomy and Cell Biology; Surgery
Record Identifier: 9985244572102771

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