Journal article
CD4+ T cells from IL-10-deficient mice transfer susceptibility to NSAID-induced Rag colitis
American journal of physiology: Gastrointestinal and liver physiology, Vol.287(2), pp.G320-G325
08/2004
DOI: 10.1152/ajpgi.00527.2003
PMID: 15246967
Abstract
Products of arachidonic acid metabolism are important for mucosal homeostasis, because blockade of this pathway with an NSAID triggers rapid onset of severe colitis in the IL-10 knockout (IL-10−/−) model of IBD. Rag mice do not make T or B cells. This study determined whether reconstitution of Rag mice with T cells from IL-10−/− mice transferred NSAID colitis susceptibility. Rag mice were reconstituted by intraperitoneal injection with splenocytes from wild-type (WT) or IL-10−/− animals. Colitis was induced by using piroxicam and was graded histologically. Isolated lamina propria mononuclear cells (LPMC), lamina propria T cells, and LPMC depleted of T cells from reconstituted Rag mice were studied for cytokine production. Only animals reconstituted with IL-10−/− CD4+ T cells and administered piroxicam developed severe colitis. LPMC from these colitic animals made IFN-γ, whose production was dependent on T cells. Some IL-10 was produced but only from non-T cells. LPMC from the healthy Rag mice that were reconstituted with WT T cells and were piroxicam resistant made much more IL-10. This was mostly T cell dependent. In conclusion, only CD4+ T cells from IL-10−/− animals leave Rag mice susceptible to NSAID-induced, Th1 colitis. Lamina propria T cells normally make large quantities of IL-10, suggesting that IL-10 from T cells may be protective.
Details
- Title: Subtitle
- CD4+ T cells from IL-10-deficient mice transfer susceptibility to NSAID-induced Rag colitis
- Creators
- Arthur M Blum - Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Ahmed Metwali - Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242David E Elliott - Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Daniel J Berg - Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Joel V Weinstock - Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology: Gastrointestinal and liver physiology, Vol.287(2), pp.G320-G325
- DOI
- 10.1152/ajpgi.00527.2003
- PMID
- 15246967
- ISSN
- 0193-1857
- eISSN
- 1522-1547
- Language
- English
- Date published
- 08/2004
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094529102771
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