Journal article
CD8+-T-Cell Response to Secreted and Nonsecreted Antigens Delivered by Recombinant Listeria monocytogenes during Secondary Infection
Infection and immunity, Vol.70(1), pp.153-162
01/2002
DOI: 10.1128/IAI.70.1.153-162.2002
PMCID: PMC127606
PMID: 11748177
Abstract
Understanding how existing antivector immunity impacts live vaccine delivery systems is critical when the same vector system may be used to deliver different antigens. We addressed the impact of antivector immunity, elicited by immunization with attenuated
actA
-deficient
Listeria monocytogenes
, on the CD8
+
-T-cell response to a well-characterized lymphocytic choriomeningitis virus epitope, NP118-126, delivered by infection with recombinant
L. monocytogenes
. Challenges of immune mice with
actA
-deficient and with wild-type recombinant
L. monocytogenes
generated similar numbers of CD8
+
T cells specific for the NP118-126 epitope. High-dose immunization with
actA
-deficient
L. monocytogenes
resulted in substantial numbers of CD8
+
T cells specific for the
L. monocytogenes
LLO91-99 epitope in the effector and memory stages of the T-cell response. Challenge of these immune mice with recombinant
L. monocytogenes
resulted in rapid control of the infection and decreased CD8
+
-T-cell responses against both the secreted and nonsecreted form of the recombinant antigen compared to the response of naïve mice. In contrast, mice immunized with a low dose of
actA
-deficient
L. monocytogenes
had ∼10-fold fewer effector and memory T cells specific for LLO91-99 and a substantially higher CD8
+
-T-cell response against the recombinant antigen after challenge with recombinant
L. monocytogenes
. Although mice immunized with low-dose
actA
-deficient
L. monocytogenes
had a substantial recall response to LLO91-99, which reached the same levels by 5 to 7 days postchallenge as that in high-dose-immunized mice, they exhibited decreased ability to control
L. monocytogenes
replication. Thus, the level of antivector immunity impacts the control of infection and efficiency of priming responses against new antigens introduced with the same vector.
Details
- Title: Subtitle
- CD8+-T-Cell Response to Secreted and Nonsecreted Antigens Delivered by Recombinant Listeria monocytogenes during Secondary Infection
- Creators
- Amy R Tvinnereim - Department of MicrobiologySara E Hamilton - Department of MicrobiologyJohn T Harty - Department of Microbiology
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.70(1), pp.153-162
- DOI
- 10.1128/IAI.70.1.153-162.2002
- PMID
- 11748177
- PMCID
- PMC127606
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 01/2002
- Academic Unit
- Pathology
- Record Identifier
- 9984047992002771
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