CD8+ T-cell epitopes within the surface glycoprotein of a neurotropic coronavirus and correlation with pathogenicity

R F Castro; S Perlman

doi:10.1128/JVI.69.12.8127-8131.1995

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CD8+ T-cell epitopes within the surface glycoprotein of a neurotropic coronavirus and correlation with pathogenicity

Journal article

Open access

Peer reviewed

CD8+ T-cell epitopes within the surface glycoprotein of a neurotropic coronavirus and correlation with pathogenicity

R F Castro and S Perlman

Journal of virology, Vol.69(12), pp.8127-8131

12/1995

DOI: 10.1128/JVI.69.12.8127-8131.1995

PMCID: PMC189767

PMID: 7494335

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https://europepmc.org/articles/pmc189767View

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Abstract

CD8+ T cells with cytotoxic activity against the surface glycoprotein (S) of mouse hepatitis virus, strain JHM, have been identified in the central nervous system (CNS) of both acutely and chronically infected C57BL/6 mice. In this report, two specific epitopes recognized by these CNS-derived cells were identified, using a panel of peptides chosen because they conformed to the allele-specific binding motif for MHC class I H-2Kb and H-2Db. The active peptides encompassed residues 510 to 518 (CSLWNGPHL, H-2Db) and 598 to 605 (RCQIFANI, H-2Kb). Both epitopes are located within the region of the S protein previously shown to be prone to deletion after passage in animals. These deleted strains are generally less neurovirulent than the wild-type virus but still are able to cause demyelination. Since C57BL/6 mice become persistently infected more commonly than many other strains of mice, these data are consistent with a role for CD8+ T-cell escape mutants in the pathogenesis of the demyelinating disease. This is the first report of CD8+ T-cell epitope localization within the S protein, the protein most strongly implicated thus far in pathogenesis in the host.

Mutagenesis

Polymerase Chain Reaction

Epitopes - analysis

Sequence Deletion

Brain - virology

Molecular Sequence Data

DNA, Viral - analysis

Viral Structural Proteins - immunology

Murine hepatitis virus - isolation & purification

Murine hepatitis virus - immunology

Base Sequence

Genes, Viral

Amino Acid Sequence

Major Histocompatibility Complex

Mice, Inbred C57BL

DNA Primers

Murine hepatitis virus - pathogenicity

Viral Structural Proteins - chemistry

Coronavirus Infections - immunology

H-2 Antigens - immunology

Animals

H-2 Antigens - chemistry

CD8-Positive T-Lymphocytes - virology

Coronavirus Infections - pathology

Brain - pathology

Mice

Mice, Inbred BALB C

CD8-Positive T-Lymphocytes - immunology

Brain - immunology

Cytotoxicity, Immunologic

Details

Title: Subtitle: CD8+ T-cell epitopes within the surface glycoprotein of a neurotropic coronavirus and correlation with pathogenicity
Creators: R F Castro - Department of Microbiology, University of Iowa, Iowa City 52242, USA
S Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.69(12), pp.8127-8131
DOI: 10.1128/JVI.69.12.8127-8131.1995
PMID: 7494335
PMCID: PMC189767
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: NS24401 / NINDS NIH HHS
Language: English
Date published: 12/1995
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777477402771

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