Journal article
CD8 T cell immunity to Plasmodium permits generation of protective antibodies after repeated sporozoite challenge
Vaccine, Vol.27(44), pp.6103-6106
2009
DOI: 10.1016/j.vaccine.2009.08.025
PMCID: PMC2759845
PMID: 19712771
Abstract
Individuals living in malaria endemic areas are subject to repeated infections yet fail to develop sterilizing immunity, however, immunization of mice with attenuated sporozoites or subunit vaccines has shown the ability to protect mice against a sporozoite challenge. We recently reported that mice primed with dendritic cells coated with the dominant circumsporozoite CD8 T cell epitope from
Plasmodium berghei followed by a boost with recombinant
Listeria monocytogenes expressing the same epitope exhibited sterile immunity against a sporozoite challenge for more than one year. In this report we show those mice do not contain protective antibodies and that depletion of CD4 T cells in the immunized mice did not affect sterile immunity. In contrast, CD8 T cell depletion eliminated protection. Thus, protective immunity generated by this immunization approach is entirely memory CD8 T cell-dependent. We also show here that mice initially protected by circumsporozoite-specific memory CD8 T cells develop sterilizing sporozoite-specific antibodies after repeated asymptomatic challenges with physiologic numbers of viable sporozoites. Therefore, initial protection by a CD8 T cell-targeted liver stage subunit vaccine allows the generation of enhanced sterilizing immune responses from repeated exposure to
Plasmodium parasites.
Details
- Title: Subtitle
- CD8 T cell immunity to Plasmodium permits generation of protective antibodies after repeated sporozoite challenge
- Creators
- Nathan W Schmidt - Department of Microbiology, University of Iowa, Iowa City, IA 52242, United StatesNoah S Butler - Department of Microbiology, University of Iowa, Iowa City, IA 52242, United StatesJohn T Harty - Department of Microbiology, University of Iowa, Iowa City, IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Vaccine, Vol.27(44), pp.6103-6106
- DOI
- 10.1016/j.vaccine.2009.08.025
- PMID
- 19712771
- PMCID
- PMC2759845
- NLM abbreviation
- Vaccine
- ISSN
- 0264-410X
- eISSN
- 1873-2518
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 2009
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984001219002771
Metrics
33 Record Views