CD8 T cell independent immunity after single dose infection-treatment-vaccination (ITV) against Plasmodium yoelii

Katherine L Doll; Noah S Butler; John T Harty

doi:10.1016/j.vaccine.2013.11.058

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CD8 T cell independent immunity after single dose infection-treatment-vaccination (ITV) against Plasmodium yoelii

Journal article

Open access

Peer reviewed

CD8 T cell independent immunity after single dose infection-treatment-vaccination (ITV) against Plasmodium yoelii

Katherine L Doll, Noah S Butler and John T Harty

Vaccine, Vol.32(4), pp.483-491

01/16/2014

DOI: 10.1016/j.vaccine.2013.11.058

PMCID: PMC3923319

PMID: 24321740

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Abstract

•P. yoelii ITV induces a larger CD8 T cell response than RAS vaccination in B6 mice.•Depletion of CD8 T cells does not abrogate ITV-induced sterilizing immunity.•ITV-induced immunity correlates with blood-stage resistance and antibodies. Sporozoite vaccination of both humans and rodents elicits potent anti-malarial immunity, but the dose of sporozoites and the number of immunizations required varies with vaccination approach. Here we examine the immunological basis for superior protection afforded from single-dose vaccination with virulent sporozoites administered under prophylatic chloroquine-cover, referred to as infection-treatment-vaccination (ITV), compared to the well-studied approach of administering radiation-attenuated Plasmodium sporozoites (RAS). Earlier rodent studies utilizing ITV and RAS vaccination suggested a major role of CD8 T cells in reducing liver parasite burden after sporozoite challenge in a BALB/c mouse model. Consistent with this, we find that in C57Bl/6 mice ITV elicits substantially higher parasite-specific CD8 T cell responses than RAS vaccination and enhances immunity against P. yoelii infection. However, we show ITV-induced CD8 T cells are not necessary for protection following liver-stage sporozoite or blood-stage parasite challenge. Mechanistically, we found protection afforded from single-dose ITV is associated with low grade, transient parasitemia shortly following cessation of chloroquine treatment and generation of potent antibody responses to blood-stage parasites. Collectively, our data show the mechanistic basis for enhanced protective immunity against P. yoelli elicited by ITV in highly susceptible C57Bl/6 mice is independent of CD8 T cells. These studies may be relevant in understanding the potent immunity observed with ITV in humans.

Subpatent infection

Antibodies

Plasmodium infections

Vaccination

CD8 T cells

Details

Title: Subtitle: CD8 T cell independent immunity after single dose infection-treatment-vaccination (ITV) against Plasmodium yoelii
Creators: Katherine L Doll - Department of Microbiology, University of Iowa, Iowa City, IA, USA
Noah S Butler - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
John T Harty - Department of Microbiology, University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Vaccine, Vol.32(4), pp.483-491
DOI: 10.1016/j.vaccine.2013.11.058
PMID: 24321740
PMCID: PMC3923319
NLM abbreviation: Vaccine
ISSN: 0264-410X
eISSN: 1873-2518
Publisher: Elsevier Ltd
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: AI85515, AI95178, AI100527; DOI: 10.13039/100000002, name: National Institutes of Health, award: AI099070
Language: English
Date published: 01/16/2014
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984001147702771

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