Journal article
CD8+ T cells specific for a single nonamer epitope of Listeria monocytogenes are protective in vivo
The Journal of experimental medicine, Vol.175(6), pp.1531-1538
06/01/1992
DOI: 10.1084/jem.175.6.1531
PMID: 1375265
Abstract
Class I major histocompatibility complex (MHC)-restricted CD8+ T cells have been demonstrated to be effective mediators of both acquired and adoptive immunity to the intracellular bacterium Listeria monocytogenes. We have recently determined that L. monocytogenes-infected H-2d mice recognize a nonamer peptide, residues 91-99, of the secreted protein listeriolysin O (LLO), in a H-2Kd-restricted fashion. In this report we have generated CD8+ T cell lines with specificity for LLO 91-99 in the context of H-2Kd by in vitro stimulation with P815 (H-2d) cells transfected with LLO. These CD8+ lines have been generated from immune donors after sublethal infection with L. monocytogenes, or after in vivo immunization with syngeneic spleen cells coated with synthetic LLO 91-99 peptide. LLO-specific CD8+ T cells derived from either protocol were capable of significant protection against L. monocytogenes infection. The in vivo protection by these CD8+ T cell lines has been shown to be solely due to recognition of LLO 91-99 in the context of H-2Kd. These studies demonstrate that CD8+ T cell immunity to a single, naturally produced peptide epitope has the potential for significant protection in a bacterial infection. Thus, the allele-specific motif approach to epitope prediction has identified a naturally produced bacterial epitope with biological relevance.
Details
- Title: Subtitle
- CD8+ T cells specific for a single nonamer epitope of Listeria monocytogenes are protective in vivo
- Creators
- John T Harty - Howard Hughes Medical Institute, University of Washington, Seattle 98195Michael J Bevan - Howard Hughes Medical Institute, University of Washington, Seattle 98195
- Resource Type
- Journal article
- Publication Details
- The Journal of experimental medicine, Vol.175(6), pp.1531-1538
- DOI
- 10.1084/jem.175.6.1531
- PMID
- 1375265
- ISSN
- 0022-1007
- eISSN
- 1540-9538
- Language
- English
- Date published
- 06/01/1992
- Academic Unit
- Pathology
- Record Identifier
- 9984046926402771
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