CFTR does not alter acidification of L cell endosomes

K V Root; J F Engelhardt; M Post; J W Wilson; R W Van Dyke

doi:10.1006/bbrc.1994.2678

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CFTR does not alter acidification of L cell endosomes

Journal article

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Peer reviewed

CFTR does not alter acidification of L cell endosomes

K V Root, J F Engelhardt, M Post, J W Wilson and R W Van Dyke

Biochemical and biophysical research communications, Vol.205(1), pp.396-401

11/30/1994

DOI: 10.1006/bbrc.1994.2678

PMID: 7528013

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Abstract

Endosomes from L cells, transduced with the CFTR gene, and the parental line, which does not express detectable levels of CFTR, were loaded with FITC-dextran, isolated and the initial rates of acidification, steady-state pHi, and proton leak rates were compared over a range of chloride concentrations (0-140 mM). Values for these parameters were similar for endosomes from both cell lines in the presence and absence of cAMP and PKA. These results indicate that CFTR does not alter L cell endosome acidification, possibly due to an adequate intrinsic CI- conductance or to a failure to incorporate sufficient functional CFTR or a necessary co-factor in endocytic membranes.

Details

Title: Subtitle: CFTR does not alter acidification of L cell endosomes
Creators: K V Root - University of Michigan
J F Engelhardt
M Post
J W Wilson
R W Van Dyke
Resource Type: Journal article
Publication Details: Biochemical and biophysical research communications, Vol.205(1), pp.396-401
DOI: 10.1006/bbrc.1994.2678
PMID: 7528013
NLM abbreviation: Biochem Biophys Res Commun
ISSN: 0006-291X
eISSN: 1090-2104
Language: English
Date published: 11/30/1994
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
Record Identifier: 9984284335202771

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