Journal article
CHOP THERAPY INDUCED MITOCHONDRIAL REDOX STATE ALTERATION IN NON-HODGKIN'S LYMPHOMA XENOGRAFTS
Journal of innovative optical health science, Vol.6(2), pp.1350011-1350011
04/01/2013
DOI: 10.1142/S1793545813500119
PMCID: PMC3672060
PMID: 23745147
Abstract
We are interested in investigating whether cancer therapy may alter the mitochondrial redox state in cancer cells to inhibit their growth and survival. The redox state can be imaged by the redox scanner that collects the fluorescence signals from both the oxidized-flavoproteins (Fp) and the reduced form of nicotinamide adenine dinucleotide (NADH) in snap-frozen tissues and has been previously employed to study tumor aggressiveness and treatment responses. Here, with the redox scanner we investigated the erects of chemotherapy on mouse xenografts of a human diffuse large B-cell lymphoma cell line (DLCL2). The mice were treated with CHOP therapy, i.e., cyclophosphamide (C) + hydroxydoxorubicin (H) + Oncovin (O) + prednisone (P) with CHO administration on day 1 and prednisone administration on days 1-5. The Fp content of the treated group was significantly decreased (p - 0: 033) on day 5, and the mitochondrial redox state of the treated group was slightly more reduced than that of the control group (p - 0: 048). The decrease of the Fp heterogeneity (measured by the mean standard deviation) had a border-line statistical significance (p - 0: 071). The result suggests that the mitochondrial metabolism of lymphoma cells was slightly suppressed and the lymphomas became less aggressive after the CHOP therapy.
Details
- Title: Subtitle
- CHOP THERAPY INDUCED MITOCHONDRIAL REDOX STATE ALTERATION IN NON-HODGKIN'S LYMPHOMA XENOGRAFTS
- Creators
- H. N. Xu - University of PennsylvaniaH. Zhao - University of PennsylvaniaT. A. Mir - University of PennsylvaniaS. C. Lee - University of PennsylvaniaM. Feng - University of PennsylvaniaR. Choe - University of RochesterJ. D. Glickson - Children's Hospital of PhiladelphiaL. Z. Li - University of Pennsylvania
- Resource Type
- Journal article
- Publication Details
- Journal of innovative optical health science, Vol.6(2), pp.1350011-1350011
- DOI
- 10.1142/S1793545813500119
- PMID
- 23745147
- PMCID
- PMC3672060
- NLM abbreviation
- J Innov Opt Health Sci
- ISSN
- 1793-5458
- eISSN
- 1793-7205
- Publisher
- World Scientific
- Number of pages
- 7
- Grant note
- 2U24-CA083105; 2R01-CA101700 / Small Animal Imaging Resources Program (SAIR) R00CA126187 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) P41RR02305; K99/R00-CA126187 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P41RR002305 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) P41EB015893 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)
- Language
- English
- Date published
- 04/01/2013
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984756260202771
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