Journal article
CHROMATIN CONDENSATION IN TERMINALLY DIFFERENTIATING MOUSE ERYTHROBLASTS DOES NOT INVOLVE SPECIAL ARCHITECTURAL PROTEINS BUT DEPENDS ON HISTONE DEACETYLATION
Chromosome research, Vol.17(1), pp.47-64
2009
DOI: 10.1007/s10577-008-9005-y
PMCID: PMC2667965
PMID: 19172406
Abstract
Terminal erythroid differentiation in vertebrates is characterized by progressive heterochromatin formation, chromatin condensation and, in mammals, culminates in nuclear extrusion. To date, although mechanisms regulating avian erythroid chromatin condensation have been identified, little is known regarding this process during mammalian erythropoiesis. To elucidate the molecular basis for mammalian erythroblast chromatin condensation, we used Friend virus-infected murine spleen erythroblasts that undergo terminal differentiation in vitro. Chromatin isolated from early and late stage erythroblasts had similar levels of linker and core histones, only a slight difference in nucleosome repeats, and no significant accumulation of known developmentally-regulated architectural chromatin proteins. However, histone H3(K9) dimethylation markedly increased while histone H4(K12) acetylation dramatically decreased and became segregated from the histone methylation as chromatin condensed. One histone deacetylase, HDAC5, was significantly upregulated during the terminal stages of Friend virus-infected erythroblast differentiation. Treatment with histone deacetylase inhibitor, trichostatin A, blocked both chromatin condensation and nuclear extrusion. Based on our data, we propose a model for a unique mechanism in which extensive histone deacetylation at pericentromeric heterochromatin mediates heterochromatin condensation in vertebrate erythroblasts that would otherwise be mediated by developmentally-regulated architectural proteins in nucleated blood cells.
Details
- Title: Subtitle
- CHROMATIN CONDENSATION IN TERMINALLY DIFFERENTIATING MOUSE ERYTHROBLASTS DOES NOT INVOLVE SPECIAL ARCHITECTURAL PROTEINS BUT DEPENDS ON HISTONE DEACETYLATION
- Creators
- Evgenya Y Popova - Biochemistry and Molecular Biology, College of Medicine, Penn State University, Hershey, PA, 17033Sharon Wald Krauss - Life Sciences Division, University of California, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720Sarah A Short - Lawrence Berkeley National LaboratoryGloria Lee - Life Sciences Division, University of California, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720Jonathan Villalobos - Life Sciences Division, University of California, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720Joan Etzell - Laboratory Medicine, University of California, San Francisco, San Francisco, CA, 94143Mark J Koury - Medicine/Hematology, TN Valley Healthcare System and Vanderbilt University, Nashville, TN, 37232Paul A Ney - St. Jude Children’s Research Hospital, Memphis, TN 38105-2794Joel Anne Chasis - Life Sciences Division, University of California, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720Sergei A Grigoryev - Biochemistry and Molecular Biology, College of Medicine, Penn State University, Hershey, PA, 17033
- Resource Type
- Journal article
- Publication Details
- Chromosome research, Vol.17(1), pp.47-64
- DOI
- 10.1007/s10577-008-9005-y
- PMID
- 19172406
- PMCID
- PMC2667965
- NLM abbreviation
- Chromosome Res
- ISSN
- 0967-3849
- eISSN
- 1573-6849
- Language
- English
- Date published
- 2009
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984070344002771
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