CIDAR MoClo: Improved MoClo Assembly Standard and New E-coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

Sonya V. Iverson; Traci L. Haddock; Jacob Beal; Douglas M. Densmore

doi:10.1021/acssynbio.5b00124

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CIDAR MoClo: Improved MoClo Assembly Standard and New E-coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

Journal article

Peer reviewed

CIDAR MoClo: Improved MoClo Assembly Standard and New E-coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

Sonya V. Iverson, Traci L. Haddock, Jacob Beal and Douglas M. Densmore

ACS synthetic biology, Vol.5(1), pp.99-103

01/15/2016

DOI: 10.1021/acssynbio.5b00124

PMID: 26479688

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Abstract

Multipart and modular DNA part libraries and assembly standards have become common tools in synthetic biology since the publication of the Gibson and Golden Gate assembly methods, yet no multipart modular library exists for use in bacterial systems. Building upon the existing MoClo assembly framework, we have developed a publicly available collection of modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in Escherichia coli. The Cross-disciplinary Integration of Design Automation Research lab (CIDAR) MoClo Library is openly available and contains promoters, ribosomal binding sites, coding sequence, terminators, vectors, and a set of fluorescent control plasmids. Optimized protocols reduce reaction time and cost by >80% from that of previously published protocols.

Biochemical Research Methods

Biochemistry & Molecular Biology

Life Sciences & Biomedicine

Science & Technology

Details

Title: Subtitle: CIDAR MoClo: Improved MoClo Assembly Standard and New E-coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology
Creators: Sonya V. Iverson - Boston University
Traci L. Haddock - Boston University
Jacob Beal - RTX
Douglas M. Densmore - Boston University
Resource Type: Journal article
Publication Details: ACS synthetic biology, Vol.5(1), pp.99-103
Publisher: Amer Chemical Soc
DOI: 10.1021/acssynbio.5b00124
PMID: 26479688
ISSN: 2161-5063
eISSN: 2161-5063
Number of pages: 5
Grant note: 1253856 / Div Of Molecular and Cellular Bioscience; National Science Foundation (NSF); NSF - Directorate for Biological Sciences (BIO) 1253856 / NSF; National Science Foundation (NSF)
Language: English
Date published: 01/15/2016
Academic Unit: Electrical and Computer Engineering
Record Identifier: 9984627208802771

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