Journal article
CL316243 treatment mitigates the inflammation in white adipose tissues of juvenile adipocyte-specific Nfe2l1 knockout mice
Free radical biology & medicine, Vol.165, pp.289-298
03/01/2021
DOI: 10.1016/j.freeradbiomed.2021.01.043
PMID: 33545311
Abstract
Nuclear factor-erythroid 2-related factor 1 (NFE2L1) is a key transcription factor that regulates cellular adaptive responses to various stresses. Our previous studies revealed that adult adipocyte-specific Nfe2l1-knockout [Nfe2l1 (f)-KO] mice show adipocyte hypertrophy and severe adipose inflammation, which can be worsened by rosiglitazone, a peroxisome proliferator-activated receptor. agonist. To further assess the crucial roles of NFE2L1 in adipocytes, we investigated the effect of CL316243, a beta 3 adrenergic agonist that promotes lipolysis via a post-translational mechanism, on adipose inflammation in juvenile Nfe2l1(f)-KO mice. In contrast to adult mice, 4-week-old juvenile Nfe2l1(f)-KO mice displayed a normal fat distribution but reduced fasting plasma glycerol levels and elevated adipocyte hypertrophy and macrophage infiltration in inguinal and gonadal WAT. In addition, Nfe2l1(f)-KO mice had decreased expression of multiple lipolytic genes and reduced lipolytic activity in WAT. While 7 days of CL316243 treatment showed no significant effect on adipose inflammation in Nfe2l1-Floxed control mice, the same treatment dramatically alleviated macrophage infiltration and mRNA expression of inflammation and pyroptosis-related genes in WAT of Nfe2l1(f)-KO mice. Together with previous findings in adult mice, the current study highlights that NFE2L1 plays a fundamental regulatory role in lipolytic gene expression and thus might be an important target to improve adipose plasticity and lipid homeostasis.
Details
- Title: Subtitle
- CL316243 treatment mitigates the inflammation in white adipose tissues of juvenile adipocyte-specific Nfe2l1 knockout mice
- Creators
- Zhendi Wang - China Medical UniversityYongyong Hou - China Medical UniversitySuping Ren - China Medical UniversityZhiyuan Liu - China Medical UniversityZhuo Zuo - China Medical UniversitySicui Huang - China Medical UniversityWanqi Wang - China Medical UniversityHuihui Wang - China Medical UniversityYanyan Chen - China Medical UniversityYuanyuan Xu - China Medical UniversityMasayuki Yamamoto - Tohoku UniversityQiang Zhang - Emory UniversityJingqi Fu - China Medical UniversityJingbo Pi - China Medical University
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.165, pp.289-298
- DOI
- 10.1016/j.freeradbiomed.2021.01.043
- PMID
- 33545311
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier
- Number of pages
- 10
- Grant note
- 2019JH8/10300012 / Liaoning Key Research and Development Guidance Plan QNZR2020019 / Educational Department of Liaoning Province 81830099; 82020108027; 81573106; 81402661; 81402635; 81400839; 81502841 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 2018YFC1311600 / National Key R&D Program of China
- Language
- English
- Date published
- 03/01/2021
- Academic Unit
- Neurology
- Record Identifier
- 9984303023302771
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