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CNC-bZIP protein NFE2L1 regulates osteoclast differentiation in antioxidant-dependent and independent manners
Journal article   Open access   Peer reviewed

CNC-bZIP protein NFE2L1 regulates osteoclast differentiation in antioxidant-dependent and independent manners

Zhiyuan Liu, Huihui Wang, Yongyong Hou, Yang Yang, Jingkun Jia, Jinzhi Wu, Zhuo Zuo, Tianchang Gao, Suping Ren, Yiying Bian, …
Redox biology, Vol.48, pp.102180-102180
12/2021
DOI: 10.1016/j.redox.2021.102180
PMCID: PMC8591424
PMID: 34763297
url
https://doi.org/10.1016/j.redox.2021.102180View
Published (Version of record) Open Access

Abstract

Fine-tuning of osteoclast differentiation (OD) and bone remodeling is crucial for bone homeostasis. Dissecting the mechanisms regulating osteoclastogenesis is fundamental to understanding the pathogenesis of various bone disorders including osteoporosis and arthritis. Nuclear factor erythroid 2-related factor 1 (NFE2L1, also known as NRF1), which belongs to the CNC-bZIP family of transcription factors, orchestrates a variety of physiological processes and stress responses. While Nfe2l1 gene may be transcribed into multiple alternatively spliced isoforms, the biological function of the different isoforms of NFE2L1 in bone metabolism, osteoclastogenesis in particular, has not been reported. Here we demonstrate that knockout of all isoforms of Nfe2l1 transcripts specifically in the myeloid lineage in mice [Nfe2l1(M)-KO] results in increased activity of osteoclasts, decreased bone mass and worsening of osteoporosis induced by ovariectomy and aging. In comparison, LysM-Cre-mediated Nfe2l1 deletion has no significant effect on the osteoblast and osteocytes. Mechanistic investigations using bone marrow cells and RAW 264.7 cells revealed that deficiency of Nfe2l1 leads to accelerated and elevated OD, which is attributed, at least in part, to enhanced accumulation of ROS in the early stage of OD and expression of nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1α (Nfatc1/α). In addition, NFE2L1 regulates the transcription of multiple antioxidant genes and Nfatc1/α and OD in an isoform-specific manner. While long isoforms of NFE2L1 function as accelerators of induction of Nfatc1/α and antioxidant genes and OD, the short isoform NFE2L1-453 serves as a brake that keeps the long isoforms’ accelerator effects in check. These findings provide a novel insight into the regulatory roles of NFE2L1 in osteoclastogenesis and highlight that NFE2L1 is essential in regulating bone remodeling and thus may be a valuable therapeutic target for bone disorders. [Display omitted] •Nfe2l1(M)-KO mice exhibit reduced bone mass and elevated osteoclast activity.•Lack of Nfe2l1 in myeloid lineage cells results in elevated osteoclastogenesis.•Lack of Nfe2l1 leads to ROS accumulation accelerating osteoclastogenesis.•Long isoforms of NFE2L1 positively regulate Nfatc1/α and osteoclastogenesis.•S-NFE2L1-453 functions as a brake on Nfatc1/α transcription and osteoclastogenesis.
 NFATc1 NFE2L1 Osteoclast Osteoclastogenesis Osteoporosis ROS

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