COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal

Peng Xu; Hannah M Hankins; Chris MacDonald; Samuel J Erlinger; Meredith N Frazier; Nicholas S Diab; Robert C Piper; Lauren P Jackson; Jason A MacGurn; Todd R Graham

doi:10.7554/eLife.28342

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COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal

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COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal

Peng Xu, Hannah M Hankins, Chris MacDonald, Samuel J Erlinger, Meredith N Frazier, Nicholas S Diab, Robert C Piper, Lauren P Jackson, Jason A MacGurn and Todd R Graham

eLife, Vol.6, e28342

10/23/2017

DOI: 10.7554/eLife.28342

PMCID: PMC5663479

PMID: 29058666

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Abstract

The COPI coat forms transport vesicles from the Golgi complex and plays a poorly defined role in endocytic trafficking. Here we show that COPI binds K63-linked polyubiquitin and this interaction is crucial for trafficking of a ubiquitinated yeast SNARE (Snc1). Snc1 is a v-SNARE that drives fusion of exocytic vesicles with the plasma membrane, and then recycles through the endocytic pathway to the Golgi for reuse in exocytosis. Removal of ubiquitin from Snc1, or deletion of a β'-COP subunit propeller domain that binds K63-linked polyubiquitin, disrupts Snc1 recycling causing aberrant accumulation in internal compartments. Moreover, replacement of the β'-COP propeller domain with unrelated ubiquitin-binding domains restores Snc1 recycling. These results indicate that ubiquitination, a modification well known to target membrane proteins to the lysosome or vacuole for degradation, can also function as recycling signal to sort a SNARE into COPI vesicles in a non-degradative pathway.

Cell Biology

Recycling

COPI

membrane trafficking

S. cerevisiae

SNARE

ubiquitin

Details

Title: Subtitle: COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal
Creators: Peng Xu - Vanderbilt University
Hannah M Hankins - Vanderbilt University
Chris MacDonald - University of Iowa
Samuel J Erlinger - Vanderbilt University
Meredith N Frazier - Vanderbilt University
Nicholas S Diab - Vanderbilt University
Robert C Piper - University of Iowa
Lauren P Jackson - Vanderbilt University
Jason A MacGurn - Vanderbilt University Medical Center
Todd R Graham - Vanderbilt University
Resource Type: Journal article
Publication Details: eLife, Vol.6, e28342
DOI: 10.7554/eLife.28342
PMID: 29058666
PMCID: PMC5663479
NLM abbreviation: Elife
ISSN: 2050-084X
eISSN: 2050-084X
Publisher: eLife Sciences Publications, Ltd
Grant note: ; 5R01GM118452 / ; 1R35GM119525 / ; 1R01GM118491 / ; 5R01GM058202 / ;
Language: English
Date published: 10/23/2017
Academic Unit: Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
Record Identifier: 9984297507102771

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