Journal article
CORP: Sources and degrees of variability in whole animal intermittent hypoxia experiments
Journal of applied physiology (1985), Vol.134(5), pp.1207-1215
05/2023
DOI: 10.1152/japplphysiol.00643.2022
PMCID: PMC10151045
PMID: 36958346
Abstract
Chamber exposures are commonly used to evaluate the physiological and pathophysiological consequences of intermittent hypoxia in animal models. Researchers in this field use both commercial and custom-built chambers in their experiments. The purpose of this Cores of Reproducibility in Physiology paper is to demonstrate potential sources of variability in these systems that researchers should consider. Evaluating the relationship between arterial oxygen saturation and inspired oxygen concentration, we found that there are important sex-dependent differences in the commonly used C57BL6/J mouse model. The time delay of the oxygen sensor that provides feedback to the system during the ramp-down and ramp-up phases was different, limiting the number of cycles per hour that can be conducted and the overall stability of the oxygen concentration. The time to reach the hypoxic and normoxic hold stages, and the overall oxygen concentration, were impacted by the cycle number. These variables were further impacted by whether there are animals present in the chamber, highlighting the importance of verifying the cycling frequency with animals in the chamber. At ≤14 cycles per hour, instability in the chamber oxygen concentration did not impact arterial oxygen saturation but may be important at higher cycle numbers. Taken together, these data demonstrate the important sources of variability that justify reporting and verifying the target oxygen concentration, cycling frequency, and arterial oxygen concentration, particularly when comparing different animal models and chamber configurations.
Details
- Title: Subtitle
- CORP: Sources and degrees of variability in whole animal intermittent hypoxia experiments
- Creators
- Zishan Zhang - Department of Internal Medicine, Division of Hematology, Oncology, and Bone Marrow Transplantation, University of Iowa, Iowa City, Iowa, United StatesHardik Kalra - Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United StatesMatthew C Delzell - A.T. Still UniversityCharles R Jedlicka - Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United StatesMikhail Vasilyev - University of IowaAnastasiia Vasileva - University of IowaMichael H Tomasson - Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United StatesMelissa L Bates - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.134(5), pp.1207-1215
- DOI
- 10.1152/japplphysiol.00643.2022
- PMID
- 36958346
- PMCID
- PMC10151045
- NLM abbreviation
- J Appl Physiol Respir Environ Exerc Physiol
- ISSN
- 0161-7567
- eISSN
- 1522-1601
- Grant note
- 134524-RSG-20-017-01-CCE / American Cancer Society (ACS) R56HL152365 / HHS | National Institutes of Health (NIH)
- Language
- English
- Electronic publication date
- 03/23/2023
- Date published
- 05/2023
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Stead Family Department of Pediatrics; Fraternal Order of Eagles Diabetes Research Center; Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984380460402771
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