Journal article
CORP: Standardizing methodology for assessing spontaneous baroreflex control of muscle sympathetic nerve activity in humans
American journal of physiology. Heart and circulatory physiology, Vol.320(2), pp.H762-H771
02/01/2021
DOI: 10.1152/AJPHEART.00704.2020
PMCID: PMC8082800
PMID: 33275522
Abstract
The use of spontaneous bursts of muscle sympathetic nerve activity (MSNA) to assess arterial baroreflex control of sympathetic nerve activity has seen increased utility in studies of both health and disease. However, methods used for analyzing spontaneous MSNA baroreflex sensitivity are highly variable across published studies. Therefore, we sought to comprehensively examine methods of producing linear regression slopes to quantify spontaneous MSNA baroreflex sensitivity in a large cohort of subjects (
= 150) to support a standardized procedure for analysis that would allow for consistent and comparable results across laboratories. The primary results demonstrated that
) consistency of linear regression slopes was considerably improved when the correlation coefficient was above -0.70, which is more stringent compared with commonly reported criterion of -0.50,
) longer recording durations increased the percentage of linear regressions producing correlation coefficients above -0.70 (1 min = 15%, 2 min = 28%, 5 min = 53%, 10 min = 67%,
< 0.001) and reaching statistical significance (1 min = 40%, 2 min = 69%, 5 min = 78%, 10 min = 89%,
< 0.001),
) correlation coefficients were improved with 3-mmHg versus 1-mmHg and 2-mmHg diastolic blood pressure (BP) bin size, and
) linear regression slopes were reduced when the acquired BP signal was not properly aligned with the cardiac cycle triggering the burst of MSNA. In summary, these results support the use of baseline recording durations of 10 min, a correlation coefficient above -0.70 for reliable linear regressions, 3-mmHg bin size, and importance of properly time-aligning MSNA and diastolic BP. Together, these findings provide best practices for determining spontaneous MSNA baroreflex sensitivity under resting conditions for improved rigor and reproducibility of results.
Details
- Title: Subtitle
- CORP: Standardizing methodology for assessing spontaneous baroreflex control of muscle sympathetic nerve activity in humans
- Creators
- Seth W Holwerda - University of KansasJason R Carter - Montana State UniversityHuan Yang - Beth Israel Deaconess Medical CenterJing Wang - The University of Texas at ArlingtonGary L Pierce - University of IowaPaul J Fadel - The University of Texas at Arlington
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.320(2), pp.H762-H771
- DOI
- 10.1152/AJPHEART.00704.2020
- PMID
- 33275522
- PMCID
- PMC8082800
- NLM abbreviation
- Am J Physiol Heart Circ Physiol
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Grant note
- P01 HL014388-48 / HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI) AA-024892 / HHS | NIH | National Institute on Alcohol Abuse and Alcoholism (NIAA) UL1 TR002537 / NCATS NIH HHS UL1TR002537 / HHS | NIH | National Center for Advancing Translational Sciences (NCATS) R01 HL127071 / NHLBI NIH HHS T32 HL007121 / NHLBI NIH HHS P01 HL014388 / NHLBI NIH HHS HL-127071 / HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI) T32HL007121 / HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI) R01 AA024892 / NIAAA NIH HHS
- Language
- English
- Date published
- 02/01/2021
- Academic Unit
- Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984267138402771
Metrics
7 Record Views