Journal article
CORRELATION BETWEEN THE STIMULATION OF C-FOS GENE-EXPRESSION AND DNA-SYNTHESIS IN RAT PANCREATIC ACINAR-CELLS IN-VITRO
Biomedical research (Tokyo), Vol.16(Suppl. 2), pp.39-44
01/01/1994
Abstract
The molecular mechanisms involved in the regulation of pancreatic acinar cell growth are unknown. In order to understand the role of c-fos expression in the effects of stimulants on pancreatic acinar cell grown we compared the effects of stimulants on DNA synthesis and c-fos gene expression in vitro. In particular we examined the effects of activating high and low affinity states of the CCK receptor on these two growth parameters. JMV-180 is a CCK analog that interacts in the rat with the high affinity state as an agonist and the low affinity state as an antagonist. CCK8 and JMV-180 each stimulated increases in DNA synthesis and c-fos gene expression. However, within the same experiment the effects of CCK were significantly greater than those of JMV-180. Furthermore, JMV-180 inhibited the maximal effects of CCK8 on both growth parameters. These data indicate that the high affinity CCK receptor state is capable of stimulating both DNA synthesis and c- fos gene expression. However, the low affinity state is also important for the full response to CCK8. With either CCK analog the effects on DNA synthesis and c-fos gene expression were well correlated. To determine the generality of these findings the effects of secretin and carbachol on growth parameters was examined. Secretin did not stimulate either parameter. In contrast, carbachol caused a large stimulation of c-fos expression and had no effect on DNA synthesis in vitro. Thus, c-fos expression may be necessary but is not sufficient for stimulation of pancreatic acinar cell growth.
Details
- Title: Subtitle
- CORRELATION BETWEEN THE STIMULATION OF C-FOS GENE-EXPRESSION AND DNA-SYNTHESIS IN RAT PANCREATIC ACINAR-CELLS IN-VITRO
- Creators
- C D LogsdonH HoshiL LU
- Resource Type
- Journal article
- Publication Details
- Biomedical research (Tokyo), Vol.16(Suppl. 2), pp.39-44
- Publisher
- BIOMED RES FOUND
- ISSN
- 0388-6107
- eISSN
- 1880-313X
- Number of pages
- 6
- Language
- English
- Date published
- 01/01/1994
- Academic Unit
- Surgery
- Record Identifier
- 9984322948802771
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