Journal article
CRISPR Base Editing in Induced Pluripotent Stem Cells
Methods in molecular biology (Clifton, N.J.), Vol.2045, pp.337-346
2019
DOI: 10.1007/7651_2019_243
PMID: 31250381
Abstract
Induced pluripotent stem cells (iPSCs) have demonstrated tremendous potential in numerous disease modeling and regenerative medicine-based therapies. The development of innovative gene transduction and editing technologies has further augmented the potential of iPSCs. Cas9-cytidine deaminases, for example, have developed as an alternative strategy to integrate single-base mutations (C → T or G → A transitions) at specific genomic loci. In this chapter, we specifically describe CRISPR (clustered regularly interspaced short palindromic repeats) base editing in iPSCs for editing precise locations in the genome. This state-of-the-art approach enables highly efficient and accurate modifications in genes. Thus, this technique not only has the potential to have biotechnology and therapeutic applications but also the ability to reveal underlying mechanisms regarding pathologies caused by specific mutations.
Details
- Title: Subtitle
- CRISPR Base Editing in Induced Pluripotent Stem Cells
- Creators
- Ya-Ju Chang - Jonas Children's Vision Care, Bernard and Shirlee Brown Glaucoma Laboratory, Columbia University, New York, NY, USAChristine L Xu - Jonas Children's Vision Care, Bernard and Shirlee Brown Glaucoma Laboratory, Columbia University, New York, NY, USAXuan Cui - Jonas Children's Vision Care, Bernard and Shirlee Brown Glaucoma Laboratory, Columbia University, New York, NY, USAAlexander G Bassuk - Department of Neurology, University of Iowa, Iowa City, IA, USAVinit B Mahajan - Omics Lab, Department of Ophthalmology, Byers Eye Institute, Stanford University, Palo Alto, CA, USAYi-Ting Tsai - Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, New York, NY, USAStephen H Tsang - Department of Pathology and Cell Biology, Columbia University, New York, NY, USA. crispr.ips@gmail.com
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.2045, pp.337-346
- Publisher
- United States
- DOI
- 10.1007/7651_2019_243
- PMID
- 31250381
- eISBN
- 9781493997138; 1493997130
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2019
- Academic Unit
- Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics)
- Record Identifier
- 9984070433002771
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