CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology

Wen-Hsuan Wu; Yi-Ting Tsai; Sally Justus; Galaxy Y Cho; Jesse D Sengillo; Yu Xu; Thiago Cabral; Chyuan-Sheng Lin; Alexander G Bassuk; Vinit B Mahajan; Stephen H Tsang

doi:10.1007/978-1-4939-7522-8_13

Journal article

CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology

Wen-Hsuan Wu, Yi-Ting Tsai, Sally Justus, Galaxy Y Cho, Jesse D Sengillo, Yu Xu, Thiago Cabral, Chyuan-Sheng Lin, Alexander G Bassuk, Vinit B Mahajan, …

Methods in molecular biology (Clifton, N.J.), Vol.1715, pp.191-205

2018

DOI: 10.1007/978-1-4939-7522-8_13

PMCID: PMC9119419

PMID: 29188514

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https://www.ncbi.nlm.nih.gov/pmc/articles/9119419View

Open Access

Abstract

CRISPR/Cas9 genome engineering is currently the leading genome surgery technology in most genetics laboratories. Combined with other complementary techniques, it serves as a powerful tool for uncovering genotype-phenotype correlations. Here, we describe a simplified protocol that was used in our publication, CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa, providing an overview of each section of the experimental process.

Genetic Engineering - methods

Retinitis Pigmentosa - genetics

Male

Cyclic Nucleotide Phosphodiesterases, Type 6 - genetics

Animals

DNA Repair

CRISPR-Cas Systems

Female

Mice

RNA, Guide

Mutation

Genetic Vectors

Disease Models, Animal

Details

Title: Subtitle: CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology
Creators: Wen-Hsuan Wu - Department of Ophthalmology, Columbia University, New York, NY, USA
Yi-Ting Tsai - Department of Ophthalmology, Columbia University, New York, NY, USA
Sally Justus - Department of Ophthalmology, Columbia University, New York, NY, USA
Galaxy Y Cho - Department of Ophthalmology, Columbia University, New York, NY, USA
Jesse D Sengillo - State University of New York Downstate Medical Center, Brooklyn, NY, USA
Yu Xu - Department of Ophthalmology, Xinhua Hospital affiliated to Shanghai Jiao Tong, University School of Medicine, Shanghai, China
Thiago Cabral - Department of Ophthalmology, Federal University of Espírito Santo (UFES), Vitória, Brazil
Chyuan-Sheng Lin - Department of Pathology & Cell Biology, Columbia University Medical Center, New York, NY, USA
Alexander G Bassuk - Department of Pediatrics and Neurology, University of Iowa, Iowa City, IA, USA
Vinit B Mahajan - Department of Ophthalmology, Byers Eye Institute, Stanford University , Palo Alto, CA, USA
Stephen H Tsang - Edward S. Harkness Eye Institute, Columbia University, New York, NY, USA. sht2@cumc.columbia.edu
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.1715, pp.191-205
DOI: 10.1007/978-1-4939-7522-8_13
PMID: 29188514
PMCID: PMC9119419
NLM abbreviation: Methods Mol Biol
eISBN: 9781493975228; 1493975226
ISSN: 1064-3745
eISSN: 1940-6029
Publisher: United States
Grant note: R01 EY025225 / NEI NIH HHS R01 EY018213 / NEI NIH HHS R01 NS098590 / NINDS NIH HHS P30 CA013696 / NCI NIH HHS R01 EY024698 / NEI NIH HHS R01 AR059703 / NIAMS NIH HHS R01 EY026682 / NEI NIH HHS K08 EY020530 / NEI NIH HHS R01 EY024665 / NEI NIH HHS P30 EY019007 / NEI NIH HHS R21 AG050437 / NIA NIH HHS
Language: English
Date published: 2018
Academic Unit: Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Neurology (Pediatrics); Neurosurgery
Record Identifier: 9984070733002771

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