Journal article
CRISPR transcriptional repression devices and layered circuits in mammalian cells
Nature methods, Vol.11(7), pp.723-U155
07/01/2014
DOI: 10.1038/NMETH.2969
PMCID: PMC4228775
PMID: 24797424
Abstract
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
Details
- Title: Subtitle
- CRISPR transcriptional repression devices and layered circuits in mammalian cells
- Creators
- Samira Kiani - Massachusetts Institute of TechnologyJacob Beal - RTXMohammad R. Ebrahimkhani - MIT, Dept Biol Engn, Cambridge, MA 02139 USAJin Huh - Korea Advanced Institute of Science and TechnologyRichard N. Hall - Massachusetts Institute of TechnologyZhen Xie - Tsinghua UniversityYinqing Li - Massachusetts Institute of TechnologyRon Weiss - Massachusetts Institute of Technology
- Resource Type
- Journal article
- Publication Details
- Nature methods, Vol.11(7), pp.723-U155
- Publisher
- Springer Nature
- DOI
- 10.1038/NMETH.2969
- PMID
- 24797424
- PMCID
- PMC4228775
- ISSN
- 1548-7091
- eISSN
- 1548-7105
- Number of pages
- 5
- Grant note
- R01CA155320 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) P50GM098792 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 2013M3A6A8073557 / Intelligent Synthetic Biology Center of Global Frontier Project - Ministry of Science, Information and Communication Technology and Future Planning of Korea 5R01CA155320-04; P50 GM098792 / US National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 07/01/2014
- Academic Unit
- Electrical and Computer Engineering
- Record Identifier
- 9984627294902771
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