Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle

F.Norman Briggs; K.Francis Lee; Joseph J Feher; Andrew S Wechsler; Kay Ohiendieck; Kevin Campbell

doi:10.1016/0014-5793(90)80025-E

Back

Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle

Journal article

Open access

Peer reviewed

Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle

F.Norman Briggs, K.Francis Lee, Joseph J Feher, Andrew S Wechsler, Kay Ohiendieck and Kevin Campbell

FEBS letters, Vol.259(2), pp.269-272

1990

DOI: 10.1016/0014-5793(90)80025-E

PMID: 2136731

Files and links (1)

url

https://doi.org/10.1016/0014-5793(90)80025-EView

Published (Version of record) Open Access

Abstract

Chronic stimulation of a predominantly fast skeletal muscle enhanced the expression of type I (slow muscle) Ca-ATPase and suppressed the expression of the type II (fast muscle) Ca-ATPase. Monoclonal antibodies IID8 and IIH11 against type I (slow) and type II (fast) isozymes respectively, were used to type the Ca-ATPases of the isolated SR (sarcoplasmic reticulum) by Western blots, and the Ca-ATPases of the muscle fibers by immunohistochemistry. Of the fibers from control muscles 80% stained for the type II isozyme and 20% for the type I isozyme. Following chronic stimulation all fibers stained for type I isozyme and none stained for type II isozyme. Ca-ATPase isozyme distribution in isolated SR confirmed this effect of chronic stimulation. The calcium uptake activities of homogenates of stimulated muscles were 22% of the control muscles. The Ca-ATPase and calcium-uptake activities of the isolated SR from stimulated muscles were, respectively, 32 and 45% of the control muscles.

Ca-ATPase isozyme: (Ruthenium red)

Skeletal muscle transformation

Sarcoplasmic reticulum

Ca uptake

Details

Title: Subtitle: Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle
Creators: F.Norman Briggs - Department of Physiology, Medical College of Virginia, Box 551, Richmond, VA 23298 USA
K.Francis Lee - Department of Surgery, Medical College of Virginia, Box 645, Richmond, VA 23298 USA
Joseph J Feher - Department of Physiology, Medical College of Virginia, Box 551, Richmond, VA 23298 USA
Andrew S Wechsler - Department of Surgery, Medical College of Virginia, Box 645, Richmond, VA 23298 USA
Kay Ohiendieck
Kevin Campbell - Department of Physiology and Biophysics, University of Iowa, Iowa City, 1A 52242, USA
Resource Type: Journal article
Publication Details: FEBS letters, Vol.259(2), pp.269-272
Publisher: Elsevier B.V
DOI: 10.1016/0014-5793(90)80025-E
PMID: 2136731
ISSN: 0014-5793
eISSN: 1873-3468
Language: English
Date published: 1990
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984068381002771

Metrics

20 Record Views

67 Times Cited - Web of Science