Journal article
Ca2+ signalling between single L-type Ca2+ channels and ryanodine receptors in heart cells
Nature (London), Vol.410(6828), pp.592-596
2001
DOI: 10.1038/35069083
PMID: 11279498
Abstract
Ca2+-induced Ca2+ release is a general mechanism that most cells use to amplify Ca2+ signals. In heart cells, this mechanism is operated between voltage-gated L-type Ca2+ channels (LCCs) in the plasma membrane and Ca2+ release channels, commonly known as ryanodine receptors, in the sarcoplasmic reticulum. The Ca2+ influx through LCCs traverses a cleft of roughly 12 nm formed by the cell surface and the sarcoplasmic reticulum membrane, and activates adjacent ryanodine receptors to release Ca2+ in the form of Ca2+ sparks. Here we determine the kinetics, fidelity and stoichiometry of coupling between LCCs and ryanodine receptors. We show that the local Ca2+ signal produced by a single opening of an LCC, named a 'Ca2+ sparklet', can trigger about 4-6 ryanodine receptors to generate a Ca2+ spark. The coupling between LCCs and ryanodine receptors is stochastic, as judged by the exponential distribution of the coupling latency. The fraction of sparklets that successfully triggers a spark is less than unity and declines in a use-dependent manner. This optical analysis of single-channel communication affords a powerful means for elucidating Ca2+-signalling mechanisms at the molecular level.
Details
- Title: Subtitle
- Ca2+ signalling between single L-type Ca2+ channels and ryanodine receptors in heart cells
- Creators
- Shi-Qiang WANG - Laboratory of Cardiovascular Sciences, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, United StatesLong-Sheng SONG - Laboratory of Cardiovascular Sciences, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, United StatesEdward G LAKATTA - Laboratory of Cardiovascular Sciences, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, United StatesHEPING CHENG - Laboratory of Cardiovascular Sciences, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, United States
- Resource Type
- Journal article
- Publication Details
- Nature (London), Vol.410(6828), pp.592-596
- Publisher
- Nature Publishing
- DOI
- 10.1038/35069083
- PMID
- 11279498
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- Language
- English
- Date published
- 2001
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984094653202771
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