Journal article
CaBP1 regulates Ca(v)1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons
Molecular and cellular neurosciences, Vol.88, pp.342-352
04/01/2018
DOI: 10.1016/j.mcn.2018.03.005
PMCID: PMC6013052
PMID: 29548764
Abstract
CaBP1 is a Ca2+ binding protein that is widely expressed in neurons in the brain, retina, and cochlea. In heterologous expression systems, CaBP1 interacts with and regulates voltage-gated Ca-v Ca2+ channels but whether this is the case in neurons is unknown. Here, we investigated the cellular functions of CaBP1 in cochlear spiral ganglion neurons (SGNs), which express high levels of CaBP1. Consistent with the role of CaBP1 as a suppressor of Ca2+-dependent inactivation (CDI) of Ca(v)1 (L-type) channels, Ca(v)1 currents underwent greater CDI in SGNs from mice lacking CaBP1 (C-KO) than in wild-type (WT) SGNs. The coupling of Ca(v)1 channels to downstream signaling pathways was also disrupted in C-KO SGNs. Activity-dependent repression of neurite growth was significantly blunted and unresponsive to Ca(v)1 antagonists in C-KO SGNs in contrast to WT SGNs. Moreover, Ca(v)1-mediated Ca2+ signals and phosphorylation of cAMP-response element binding protein were reduced in C KO SGNs compared to WT SGNs. Our findings establish a role for CaBP1 as an essential regulator of Ca(v)1 channels in SGNs and their coupling to downstream pathways controlling activity-dependent transcription and neurite growth.
Details
- Title: Subtitle
- CaBP1 regulates Ca(v)1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons
- Creators
- Tian Yang - University of IowaJi-Eun Choi - University of IowaDaniel Soh - University of IowaKevin Tobin - University of IowaMei-ling Joiner - University of IowaMarlan Hansen - University of IowaAmy Lee - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular neurosciences, Vol.88, pp.342-352
- DOI
- 10.1016/j.mcn.2018.03.005
- PMID
- 29548764
- PMCID
- PMC6013052
- NLM abbreviation
- Mol Cell Neurosci
- ISSN
- 1044-7431
- eISSN
- 1095-9327
- Publisher
- Elsevier
- Number of pages
- 11
- Grant note
- NS084190; DC009433; DC010362 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01NS084190 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) Carver Research Program of Excellence Award MR130438 / Department of Defense; United States Department of Defense R55DC009433 / NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD)
- Language
- English
- Date published
- 04/01/2018
- Academic Unit
- Molecular Physiology and Biophysics; Biology; Neurosurgery; Otolaryngology
- Record Identifier
- 9984297613202771
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