Journal article
CaCO 3 Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma
Journal of dental research, Vol.103(2), pp.147-155
02/2024
DOI: 10.1177/00220345231216110
PMCID: PMC10915176
PMID: 38149503
Abstract
MicroRNA (miR)–200c suppresses the initiation and progression of oral squamous cell carcinoma (OSCC), the most prevalent head and neck cancer with high recurrence, metastasis, and mortality rates. However, miR-200c–based gene therapy to inhibit OSCC growth has yet to be reported. To develop an miR-based gene therapy to improve the outcomes of OSCC treatment, this study investigates the feasibility of plasmid DNA (pDNA) encoding miR-200c delivered via nonviral CaCO3-based nanoparticles to inhibit OSCC tumor growth. CaCO3-based nanoparticles with various ratios of CaCO3 and protamine sulfate (PS) were used to transfect pDNA encoding miR-200c into OSCC cells, and the efficiency of these nanoparticles was evaluated. The proliferation, migration, and associated oncogene production, as well as in vivo tumor growth for OSCC cells overexpressing miR-200c, were also quantified. It was observed that, while CaCO3-based nanoparticles improve transfection efficiencies of pDNA miR-200c, the ratio of CaCO3 to PS significantly influences the transfection efficiency. Overexpression of miR-200c significantly reduced proliferation, migration, and oncogene expression of OSCC cells, as well as the tumor size of cell line–derived xenografts (CDX) in mice. In addition, a local administration of pDNA miR-200c using CaCO3 delivery significantly enhanced miR-200c transfection and suppressed tumor growth of CDX in mice. These results strongly indicate that the nanocomplexes of CaCO3/pDNA miR-200c may potentially be used to reduce oral cancer recurrence and improve clinical outcomes in OSCC treatment, while more comprehensive examinations to confirm the safety and efficacy of the CaCO3/pDNA miR-200c system using various preclinical models are needed.
Details
- Title: Subtitle
- CaCO 3 Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma
- Creators
- Q J Ding - Iowa Institute for Oral Health Research, College of Dentistry, University of Iowa, Iowa City, IA, USAM T Remy - University of IowaC Upara - University of IowaJ Hu - University of IowaA V Mora Mata - Department of Chemistry, College of Liberal Arts and Sciences, University of Iowa, Iowa City, IA, USAA J Haes - Department of Chemistry, College of Liberal Arts and Sciences, University of Iowa, Iowa City, IA, USAE Lanzel - Department of Oral Pathology, Radiology, & Medicine, College of Dentistry, University of Iowa, Iowa City, Iowa, IA, USAH Sun - Iowa Institute for Oral Health Research, College of Dentistry, University of Iowa, Iowa City, IA, USAM R Buchakjian - Department of Otolaryngology-Head and Neck Surgery, Carver College of Medicine, University of Iowa, Iowa City, IA, USAL Hong - Iowa Institute for Oral Health Research, College of Dentistry, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.103(2), pp.147-155
- DOI
- 10.1177/00220345231216110
- PMID
- 38149503
- PMCID
- PMC10915176
- NLM abbreviation
- J Dent Res
- eISSN
- 1544-0591
- Grant note
- DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: F31DE031153; DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: R01DE026433; DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: R01DE029159; DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: T90DE023520
- Language
- English
- Electronic publication date
- 12/27/2023
- Date published
- 02/2024
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Oral Pathology, Radiology and Medicine; Prosthodontics; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Chemistry; Dental Research; Otolaryngology
- Record Identifier
- 9984536733702771
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