Journal article
Calcium Flickers Steer Cell Migration
Nature (London), Vol.457(7231), pp.901-905
02/12/2009
DOI: 10.1038/nature07577
PMCID: PMC3505761
PMID: 19118385
Abstract
Directional movement is a property common to all cell types during development and is critical to tissue remodelling and regeneration after damage
1
–
3
. In migrating cells, calcium plays a multifunctional role in directional sensing, cytoskeleton redistribution, traction force generation, and relocation of focal adhesions
1
,
4
–
7
. Here we visualise, for the first time, high-calcium microdomains (“calcium flickers”), and their patterned activation in migrating fibroblasts. Calcium flicker activity is dually coupled to membrane tension (
via
TRPM7, a stretch-activated Ca
2+
-permeant channel of the transient receptor potential superfamily
8
) and chemoattractant signal transduction (
via
type 2 inositol 1,4,5-trisphosphate receptors). Interestingly, calcium flickers are most active at the leading lamella of migrating cells, displaying a 4:1 front-to-rear polarisation opposite to the global calcium gradient
6
. When exposed to a PDGF gradient perpendicular to cell movement, asymmetric calcium flicker activity develops across the lamella and promotes the turning of migrating fibroblasts. These findings illustrate how the exquisite spatiotemporal organisation of calcium microdomains can orchestrate complex cellular processes such as cell migration.
Details
- Title: Subtitle
- Calcium Flickers Steer Cell Migration
- Creators
- Chaoliang Wei - Institute of Molecular Medicine, National Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, ChinaLong-Sheng Song - Department of Internal Medicine, Division of Cardiovascular Medicine, University of Iowa Carver College of Medicine, Iowa City 52242, Iowa, USAXianhua Wang - Institute of Molecular Medicine, National Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, ChinaMin Chen - Institute of Molecular Medicine, National Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, ChinaKunfu Ouyang - Institute of Molecular Medicine, National Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, ChinaHeping Cheng - Institute of Molecular Medicine, National Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, China
- Resource Type
- Journal article
- Publication Details
- Nature (London), Vol.457(7231), pp.901-905
- DOI
- 10.1038/nature07577
- PMID
- 19118385
- PMCID
- PMC3505761
- NLM abbreviation
- Nature
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- Grant note
- R01 HL090905 || HL / National Heart, Lung, and Blood Institute : NHLBI
- Language
- English
- Date published
- 02/12/2009
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984094657502771
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