Journal article
Calcium and phosphate supplementation promotes bone cell mineralization: Implications for hydroxyapatite (HA)‐enhanced bone formation
Journal of biomedical materials research, Vol.52(2), pp.270-278
11/2000
DOI: 10.1002/1097-4636(200011)52:2<270::AID-JBM5>3.0.CO;2-1
PMID: 10951365
Abstract
Organic phosphate, in particular beta‐glycerophosphate (β‐GP), has been used to induce mineralization in cell culture systems. It serves as a source of inorganic phosphate when hydrolyzed by alkaline phosphatase. This study examined the effect of supplemental calcium and phosphate as well as the influence of various metabolic inhibitors on mineralization in a rat osteoblast‐like cell‐culture system. Mineralization was induced by supplementation of 1.8 mM of Ca+2 and 5 mM of β‐GP or Pi. Mineral deposits associated with in vitro mineralization were revealed under SEM and TEM. Levamisole (10–100 μM) inhibited alkaline phosphatase activity and effectively reduced mineral formation. Actinomycin (500 ng/mL) and cycloheximide (50 μg/mL) also reduced mineral depositions by blocking RNA synthesis and protein synthesis, respectively. Levamisole and β‐GP did not appear to influence DNA synthesis. Spontaneous precipitation of calcium phosphate mineral was not detected in the culture medium with calcium and phosphate supplements in the absence of cell culture. The findings suggest that an elevated concentration of calcium and phosphate is crucial for in vitro mineralization. Furthermore, the mineralization process is associated with biologic events rather than with a spontaneous precipitation of calcium phosphate mineral. In view of the degradation potential of hydroxyapatite (HA)‐coated implants, these results may be a viable indication that HA enhances bone formation through a similar mechanism. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 52, 270–278, 2000.
Details
- Title: Subtitle
- Calcium and phosphate supplementation promotes bone cell mineralization: Implications for hydroxyapatite (HA)‐enhanced bone formation
- Creators
- Yu‐Liang ChangClark M StanfordJohn C Keller
- Resource Type
- Journal article
- Publication Details
- Journal of biomedical materials research, Vol.52(2), pp.270-278
- DOI
- 10.1002/1097-4636(200011)52:2<270::AID-JBM5>3.0.CO;2-1
- PMID
- 10951365
- NLM abbreviation
- J Biomed Mater Res
- ISSN
- 0021-9304
- eISSN
- 1097-4636
- Publisher
- John Wiley & Sons, Inc; New York
- Number of pages
- 9
- Grant note
- NIDR/NIH (DE08540) Oral and Maxillofacial Surgery Foundation
- Language
- English
- Date published
- 11/2000
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Graduate College Admin and Gen; President; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
- Record Identifier
- 9984066100002771
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