Journal article
Calcium-dependent regulation of airway epithelial chloride channels
American journal of physiology. Lung cellular and molecular physiology, Vol.258(2), pp.L25-L32
02/01/1990
DOI: 10.1152/ajplung.1990.258.2.L25
PMID: 1689551
Abstract
To determine how cell calcium ([Ca2+]c) regulates apical Cl- channels, we measured the rate of 125-Iodide (125I-) efflux to assay Cl- channel activity in intact cells and examined cell-free membrane patches from cultured canine tracheal epithelial cells. The Ca2+ elevating agonist bradykinin and the calcium ionophore A23187 increased 125I- efflux. This response did not require prostaglandin production. Under several conditions, changes in [Ca2+]c were temporally dissociated from changes in channel activation: a transient increase in [Ca2+]c caused a prolonged stimulation of 125I- efflux. Neither Cl- channel activation nor open-channel probability was affected by varying internal [Ca2+] in excised membrane patches. Adenosine 3',5'-cyclic monophosphate (cAMP)- and Ca2(+)-dependent channel activation may be independent: cAMP-stimulated 125I- efflux did not require an increase in [Ca2+]c, Ca2(+)-stimulated efflux did not require an increase in cAMP, and simultaneous addition of A23187 and isoproterenol produced additive effects on 125I- efflux. The data suggest that an increase in [Ca2+]c activates Cl- channels, however, the effect of Ca2+ appears to be indirect, not involving a ligand-type interaction with the channel.
Details
- Title: Subtitle
- Calcium-dependent regulation of airway epithelial chloride channels
- Creators
- John P Clancy - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242John D McCann - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242Ming Li - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242Michael J Welsh - Howard Hughes Medical Institute, Department of Internal Medicine,University of Iowa College of Medicine, Iowa City 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.258(2), pp.L25-L32
- DOI
- 10.1152/ajplung.1990.258.2.L25
- PMID
- 1689551
- NLM abbreviation
- Am J Physiol Lung Cell Mol Physiol
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Publisher
- American Physiological Society
- Language
- English
- Date published
- 02/01/1990
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020702602771
Metrics
20 Record Views