Calcium fluxes in dorsal forerunner cells antagonize beta-catenin and alter left-right patterning

Igor Schneider; Douglas W Houston; Michael R Rebagliati; Diane C Slusarski

doi:10.1242/dev.004713

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Calcium fluxes in dorsal forerunner cells antagonize beta-catenin and alter left-right patterning

Journal article

Peer reviewed

Calcium fluxes in dorsal forerunner cells antagonize beta-catenin and alter left-right patterning

Igor Schneider, Douglas W Houston, Michael R Rebagliati and Diane C Slusarski

Development (Cambridge), Vol.135(1), pp.75-84

01/2008

DOI: 10.1242/dev.004713

PMID: 18045845

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Abstract

Establishment of the left-right axis is essential for normal organ morphogenesis and function. Ca(2+) signaling and cilia function in the zebrafish Kuppfer's Vesicle (KV) have been implicated in laterality. Here we describe an endogenous Ca(2+) release event in the region of the KV precursors (dorsal forerunner cells, DFCs), prior to KV and cilia formation. Manipulation of Ca(2+) release to disrupt this early flux does not impact early DFC specification, but results in altered DFC migration or cohesion in the tailbud at somite stages. This leads to disruption of KV formation followed by bilateral expression of asymmetrical genes, and randomized organ laterality. We identify beta-catenin inhibition as a Ca(2+)-signaling target and demonstrate that localized loss of Ca(2+) within the DFC region or DFC-specific activation of beta-catenin is sufficient to alter laterality in zebrafish. We identify a previously unknown DFC-like cell population in Xenopus and demonstrate a similar Ca(2+)-sensitive stage. As in zebrafish, manipulation of Ca(2+) release results in ectopic nuclear beta-catenin and altered laterality. Overall, our data support a conserved early Ca(2+) requirement in DFC-like cell function in zebrafish and Xenopus.

Calcium - metabolism

Xenopus - genetics

Embryo, Nonmammalian - metabolism

Embryo, Nonmammalian - embryology

Zebrafish - embryology

Xenopus - embryology

beta Catenin - metabolism

Zebrafish - genetics

Calcium - antagonists & inhibitors

Animals

Cell Nucleus - metabolism

Xenopus - metabolism

Gene Expression Regulation, Developmental

beta Catenin - antagonists & inhibitors

Zebrafish - metabolism

Body Patterning

Calcium Signaling

Details

Title: Subtitle: Calcium fluxes in dorsal forerunner cells antagonize beta-catenin and alter left-right patterning
Creators: Igor Schneider - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
Douglas W Houston
Michael R Rebagliati
Diane C Slusarski
Resource Type: Journal article
Publication Details: Development (Cambridge), Vol.135(1), pp.75-84
DOI: 10.1242/dev.004713
PMID: 18045845
NLM abbreviation: Development
ISSN: 0950-1991
eISSN: 1477-9129
Publisher: England
Grant note: CA112369 / NCI NIH HHS R01 CA112369 / NCI NIH HHS
Language: English
Date published: 01/2008
Academic Unit: Microbiology and Immunology; Iowa Neuroscience Institute; Biology
Record Identifier: 9983992099602771

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