Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons

Josue A Lopez; Annamarie Yamamoto; Joseph T Vecchi; Jussara Hagen; Kyungmoo Lee; Milan Sonka; Marlan R Hansen; Amy Lee

doi:10.1038/s41598-023-29622-9

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Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons

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Open access

Peer reviewed

Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons

Josue A Lopez, Annamarie Yamamoto, Joseph T Vecchi, Jussara Hagen, Kyungmoo Lee, Milan Sonka, Marlan R Hansen and Amy Lee

Scientific reports, Vol.13(1), 2608

02/14/2023

DOI: 10.1038/s41598-023-29622-9

PMCID: PMC9929226

PMID: 36788334

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Abstract

Caldendrin is a Ca2+ binding protein that interacts with multiple effectors, such as the Cav1 L-type Ca2+ channel, which play a prominent role in regulating the outgrowth of dendrites and axons (i.e., neurites) during development and in response to injury. Here, we investigated the role of caldendrin in Cav1-dependent pathways that impinge upon neurite growth in dorsal root ganglion neurons (DRGNs). By immunofluorescence, caldendrin was localized in medium- and large- diameter DRGNs. Compared to DRGNs cultured from WT mice, DRGNs of caldendrin knockout (KO) mice exhibited enhanced neurite regeneration and outgrowth. Strong depolarization, which normally represses neurite growth through activation of Cav1 channels, had no effect on neurite growth in DRGN cultures from female caldendrin KO mice. Remarkably, DRGNs from caldendrin KO males were no different from those of WT males in terms of depolarization-dependent neurite growth repression. We conclude that caldendrin opposes neurite regeneration and growth, and this involves coupling of Cav1 channels to growth-inhibitory pathways in DRGNs of females but not males.

Animals

Axons - metabolism

Cells, Cultured

Female

Ganglia, Spinal

Mice

Nerve Regeneration

Neurites - metabolism

Neurons - metabolism

Details

Title: Subtitle: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
Creators: Josue A Lopez - The University of Texas at Austin
Annamarie Yamamoto - Department of Neuroscience, University of Texas-Austin, 100 E. 24th St., Austin, TX, 78712, USA
Joseph T Vecchi - University of Iowa
Jussara Hagen - University of Iowa
Kyungmoo Lee - University of Iowa
Milan Sonka - University of Iowa
Marlan R Hansen - University of Iowa
Amy Lee - Department of Neuroscience, University of Texas-Austin, 100 E. 24th St., Austin, TX, 78712, USA. amy.lee1@austin.utexas.edu
Resource Type: Journal article
Publication Details: Scientific reports, Vol.13(1), 2608
DOI: 10.1038/s41598-023-29622-9
PMID: 36788334
PMCID: PMC9929226
NLM abbreviation: Sci Rep
eISSN: 2045-2322
Grant note: T32-GM007337 / NIH HHS R01-DC012578 / NIH HHS T32NS045549 / NIH HHS R01-EB004640 / NIH HHS R01-EY026817 / NIH HHS
Language: English
Date published: 02/14/2023
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Molecular Physiology and Biophysics; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Injury Prevention Research Center; Neurosurgery; Otolaryngology; Ophthalmology and Visual Sciences
Record Identifier: 9984367457502771

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