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Caloric restriction improves thermotolerance and reduces hyperthermia‐induced cellular damage in old rats
Journal article   Peer reviewed

Caloric restriction improves thermotolerance and reduces hyperthermia‐induced cellular damage in old rats

D. M HALL, T. D OBERLEY, P. M MOSELEY, G. R BUETTNER, L. W OBERLEY, R WEINDRUCH and K. C KREGEL
The FASEB journal, Vol.14(1), pp.78-86
01/2000
DOI: 10.1096/fasebj.14.1.78
PMID: 10627282

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Abstract

ABSTRACT Adult‐onset, long‐term caloric restriction (CR) prolongs maximum life span in laboratory rodents. However, the effect of this intervention on an organism's ability to cope with a physical challenge has not been explored. We investigated the influence of CR and aging on stress tolerance in old rats exposed to an environmental heating protocol on two consecutive days. We hypothesized that CR would increase heat tolerance by reducing cellular stress and subsequent accrual of oxidative injury. All calorically restricted rats survived both heat exposures compared with only 50% of their control‐fed counterparts. CR also decreased heat‐induced radical generation, stress protein accumulation, and cellular injury in the liver. In addition, heat stress stimulated marked induction of the antioxidant enzymes manganese‐containing superoxide dismutase and catalase, along with strong nuclear catalase expression in liver samples from rats subjected to CR. In contrast, stress‐related induction of antioxidant enzymes was blunted, and nuclear catalase expression was unchanged from euthermic conditions in the control‐fed group. These data suggest that CR reduces cellular injury and improves heat tolerance of old animals by lowering radical production and preserving cellular ability to adapt to stress through antioxidant enzyme induction and translocation of these proteins to the nucleus.—Hall, D. M., Oberley, T. D., Moseley, P. M., Buettner, G. R., Oberley, L. W., Weindruch, R., Kregel, K. C. Caloric restriction improves thermotolerance and reduces hyperthermia‐induced cellular damage in old rats. FASEB J. 14, 78–86(2000)
Aging Oxidative Stress stress proteins free radicals HSP70 antioxidant enzymes

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