Journal article
Calpain-5 gene expression in the mouse eye and brain
BMC research notes, Vol.10(1), pp.602-602
11/21/2017
DOI: 10.1186/s13104-017-2927-8
PMCID: PMC5697233
PMID: 29157313
Abstract
Objective: Our objective was to characterize CAPN5 gene expression in the mouse central nervous system. Mouse brain and eye sections were probed with two high-affinity RNA oligonucleotide analogs designed to bind CAPN5 RNA and one scramble, control oligonucleotide. Images were captured in brightfield.
Results: CAPN5 RNA probes were validated on mouse breast cancer tumor tissue. In the eye, CAPN5 was expressed in the ganglion cell, inner nuclear and outer nuclear layers of the retina. Signal could not be detected in the ciliary body or the iris because of the high density of melanin. In the brain, CAPN5 was expressed in the granule cell layers of the hippocampus and cerebellum. There was scattered expression in pons. The visual cortex showed faint signal. Most signal in the brain was in a punctate pattern.
Details
- Title: Subtitle
- Calpain-5 gene expression in the mouse eye and brain
- Creators
- Kellie Schaefer - Palo Alto, CA 94304 USAMaryAnn Mahajan - Palo Alto, CA 94304 USAAnuradha Gore - Palo Alto, CA 94304 USAStephen H Tsang - New York, NY USAAlexander G Bassuk - Iowa City, IA USAVinit B Mahajan - Palo Alto, CA 94304 USA
- Resource Type
- Journal article
- Publication Details
- BMC research notes, Vol.10(1), pp.602-602
- DOI
- 10.1186/s13104-017-2927-8
- PMID
- 29157313
- PMCID
- PMC5697233
- NLM abbreviation
- BMC Res Notes
- ISSN
- 1756-0500
- eISSN
- 1756-0500
- Publisher
- BioMed Central; London
- Grant note
- ; C-NY05-0705-0312 / ; R21AG050437 / ; F31EY026789; R01EY026682; R01EY024665; R01EY025225; R01EY024698 / ;
- Language
- English
- Date published
- 11/21/2017
- Academic Unit
- Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics)
- Record Identifier
- 9984070345502771
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