Journal article
Campylobacter jejuni induces differentiation of human neutrophils to the CD16hi/CD62Llo subtype
Journal of leukocyte biology, Vol.112(6), pp.1457-1470
12/2022
DOI: 10.1002/JLB.4A0322-155RR
PMID: 35866361
Abstract
The discovery of neutrophil subtypes has expanded what is known about neutrophil functions, yet there is still much to learn about the role of these subtypes during bacterial infection. We investigated whether Campylobacter jejuni induced differentiation of human neutrophils into the hypersegmented, CD16(hi)/CD62L(lo) subtype. In addition, we investigated whether C. jejuni-dependent differentiation of this neutrophil subtype induced cancer-promoting activities of human T cells and colonocytes, which were observed in other studies of hypersegmented, CD16(hi)/CD62L(lo) neutrophils. We found that C. jejuni causes a significant shift in human neutrophil populations to the hypersegmented, CD16(hi)/CD62L(lo) subtype and that those populations exhibit delayed apoptosis, elevated arginase-1 expression, and increased reactive oxygen species production. Furthermore, incubation of C. jejuni-infected neutrophils with human T cells resulted in decreased expression of the zeta-chain of the TCR, which was restored upon supplementation with exogenous L-arginine. In addition, incubation of C. jejuni-infected neutrophils with human colonocytes resulted in increased HIF-1 alpha stabilization and NF-kappa B activation in those colonocytes, which may result in the up-regulation of protumorigenic genes.
Details
- Title: Subtitle
- Campylobacter jejuni induces differentiation of human neutrophils to the CD16hi/CD62Llo subtype
- Creators
- Carolina G. Dolislager - University of Tennessee at KnoxvilleSean M. Callahan - University of Tennessee at KnoxvilleDallas R. Donohoe - University of Tennessee at KnoxvilleJeremiah G. Johnson - University of Tennessee at Knoxville
- Resource Type
- Journal article
- Publication Details
- Journal of leukocyte biology, Vol.112(6), pp.1457-1470
- Publisher
- Oxford Univ Press
- DOI
- 10.1002/JLB.4A0322-155RR
- PMID
- 35866361
- ISSN
- 0741-5400
- eISSN
- 1938-3673
- Number of pages
- 14
- Grant note
- UTK start-up funds 2019-67017-29261 / USDA NIFA; United States Department of Agriculture (USDA)
- Language
- English
- Date published
- 12/2022
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696562802771
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