Journal article
Can Myoglobin Expression in Pancreatic Beta Cells Improve Insulin Secretion Under Hypoxia? An Exploratory Study With Transgenic Porcine Islets
Artificial organs, Vol.31(7), pp.521-531
Received June 2006; revised November 2006.
07/2007
DOI: 10.1111/j.1525-1594.2007.00416.x
PMID: 17584476
Abstract
The feasibility of myoglobin (Mb)-facilitated oxygen transport in improving porcine islet survival under hypoxia was investigated. Discrete groups of islets were transfected with replication-defective adenoviral vector Ad5 respiratory syncitial virus (RSV) to induce expression of Mb or green fluorescent protein (GFP). Native islets served as the controls. In vitro studies at 37°C assessed islet insulin secretion efficacy: (i) to a glucose challenge from 30 to 300 mg/dL at fixed pO2; and (ii) at variable oxygen tensions ranging from 5 to 40 mm Hg over 12 h. The transfection was effective in initiating islet expression of Mb or GFP. Low Mb-expression levels equivalent to 2% the Mb concentration in a muscle cell (0.25 ng of Mb per islet) were documented, with no statistical improvement in insulin secretion. A surprising side note is that insulin secretion was impaired in islets expressing GFP. Improved Mb expression is essential to determine the feasibility of enhancing islet survival under hypoxia.
Details
- Title: Subtitle
- Can Myoglobin Expression in Pancreatic Beta Cells Improve Insulin Secretion Under Hypoxia? An Exploratory Study With Transgenic Porcine Islets
- Creators
- Himantha Kushanie Tilakaratne - Department of Chemical and Biochemical Engineering, The University of IowaBaoli Yang - University of Iowa, BioVentures CenterStephen K. Hunter - University of Iowa, Obstetrics and GynecologyMark E. AndrackiVictor G.J. Rodgers - Department of Chemical and Biochemical Engineering, The University of Iowa
- Resource Type
- Journal article
- Publication Details
- Artificial organs, Vol.31(7), pp.521-531
- Edition
- Received June 2006; revised November 2006.
- Publisher
- Blackwell Publishing Inc
- DOI
- 10.1111/j.1525-1594.2007.00416.x
- PMID
- 17584476
- ISSN
- 0160-564X
- eISSN
- 1525-1594
- Number of pages
- 11
- Language
- English
- Date published
- 07/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; BioVentures Center; Obstetrics and Gynecology
- Record Identifier
- 9983557493602771
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