Canal cristae growth and fiber extension to the outer hair cells of the mouse ear require Prox1 activity

Bernd Fritzsch; Miriam Dillard; Alfonso Lavado; Natasha L Harvey; Israt Jahan

doi:10.1371/journal.pone.0009377

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Canal cristae growth and fiber extension to the outer hair cells of the mouse ear require Prox1 activity

Journal article

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Peer reviewed

Canal cristae growth and fiber extension to the outer hair cells of the mouse ear require Prox1 activity

Bernd Fritzsch, Miriam Dillard, Alfonso Lavado, Natasha L Harvey and Israt Jahan

PloS one, Vol.5(2), pp.e9377-e9377

02/23/2010

DOI: 10.1371/journal.pone.0009377

PMCID: PMC2826422

PMID: 20186345

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Abstract

The homeobox gene Prox1 is required for lens, retina, pancreas, liver, and lymphatic vasculature development and is expressed in inner ear supporting cells and neurons. We have investigated the role of Prox1 in the developing mouse ear taking advantage of available standard and conditional Prox1 mutant mouse strains using Tg(Pax2-Cre) and Tg(Nes-Cre). A severe reduction in the size of the canal cristae but not of other vestibular organs or the cochlea was identified in the E18.5 Prox1(Flox/Flox); Tg(Pax2-Cre) mutant ear. In these mutant embryos, hair cell differentiated; however, their distribution pattern was slightly disorganized in the cochlea where the growth of type II nerve fibers to outer hair cells along Prox1 expressing supporting cells was severely disrupted. In the case of Nestin-Cre, we found that newborn Prox1(Flox/Flox); Tg(Nestin-Cre) exhibit only a disorganized innervation of outer hair cells despite apparently normal cellular differentiation of the organ of Corti, suggesting a cell-autonomous function of Prox1 in neurons. These results identify a dual role of Prox1 during inner ear development; growth of the canal cristae and fiber guidance of Type II fibers along supporting cells in the cochlea.

Immunohistochemistry

Cochlea - embryology

Ear, Inner - embryology

Homeodomain Proteins - metabolism

Male

Vestibule, Labyrinth - metabolism

Embryo, Mammalian - metabolism

Epithelium - embryology

In Situ Hybridization

Time Factors

Cochlea - metabolism

Gene Expression Regulation, Developmental

Tumor Suppressor Proteins - genetics

Ear, Inner - ultrastructure

Female

Vestibule, Labyrinth - embryology

Cell Differentiation

Animals, Newborn

Microscopy, Electron, Transmission

Tumor Suppressor Proteins - metabolism

Epithelium - metabolism

Mice, Transgenic

Homeodomain Proteins - genetics

Mice, Knockout

Pregnancy

Microscopy, Confocal

Ear, Inner - metabolism

Animals

Hair Cells, Auditory, Outer - metabolism

Embryo, Mammalian - embryology

Mice

Mutation

Details

Title: Subtitle: Canal cristae growth and fiber extension to the outer hair cells of the mouse ear require Prox1 activity
Creators: Bernd Fritzsch - Department of Biology, University of Iowa, Iowa City, Iowa, United States of America. bernd-fritzsch@uiowa.edu
Miriam Dillard
Alfonso Lavado
Natasha L Harvey
Israt Jahan
Resource Type: Journal article
Publication Details: PloS one, Vol.5(2), pp.e9377-e9377
DOI: 10.1371/journal.pone.0009377
PMID: 20186345
PMCID: PMC2826422
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: R01 DC005590-07 / NIDCD NIH HHS R01 DC005590 / NIDCD NIH HHS R01-DC005590 / NIDCD NIH HHS
Language: English
Date published: 02/23/2010
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070262702771

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