Journal article
Canine glyceraldehyde-3-phosphate dehydrogenase complementary DNA: polymerase chain reaction amplification, cloning, partial sequence analysis, and use as loading control in ribonuclease protection assays
American journal of veterinary research, Vol.57(3), pp.254-257
03/1996
PMID: 8669750
Abstract
To use canine glyceraldehyde-3-phosphate dehydrogenase complementary DNA (GAPDH cDNA) as a template in ribonuclease (RNase) protection assays to measure canine GAPDH mRNA expression.
Primers designed from the human GAPDH gene were used to amplify a 191-base pair canine GAPDH cDNA by reverse-transcription polymerase chain reaction. The cDNA was sequenced, and used as a template for RNase protection assay.
Total RNA was isolated from a canine squamous carcinoma cell line.
Canine GAPDH cDNA had a high degree of homology to human, rat, and mouse GAPDH. In vitro transcription of canine GAPDH cDNA was used to produce complementary RNA that detected canine GAPDH mRNA by RNase protection assay.
Canine GAPDH cDNA is a useful loading control to be used in RNase protection assays measuring mRNA expression in canine cells or tissues.
Details
- Title: Subtitle
- Canine glyceraldehyde-3-phosphate dehydrogenase complementary DNA: polymerase chain reaction amplification, cloning, partial sequence analysis, and use as loading control in ribonuclease protection assays
- Creators
- A Gröne - Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus 43210, USAM T WeckmannC C CapenT J Rosol
- Resource Type
- Journal article
- Publication Details
- American journal of veterinary research, Vol.57(3), pp.254-257
- Publisher
- United States
- PMID
- 8669750
- ISSN
- 0002-9645
- eISSN
- 1943-5681
- Grant note
- AR40220 / NIAMS NIH HHS AR01923 / NIAMS NIH HHS
- Language
- English
- Date published
- 03/1996
- Academic Unit
- Psychiatry; Family and Community Medicine
- Record Identifier
- 9984025277102771
Metrics
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