Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Zhongjia Jiang; Fuyang Song; Yanan Li; Di Xue; Guangcun Deng; Min Li; Xiaoming Liu; Yujiong Wang

doi:10.1155/2017/9891673

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Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

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Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Zhongjia Jiang, Fuyang Song, Yanan Li, Di Xue, Guangcun Deng, Min Li, Xiaoming Liu and Yujiong Wang

Mediators of inflammation, Vol.2017, pp.9891673-9891673

2017

DOI: 10.1155/2017/9891673

PMCID: PMC5434471

PMID: 28553017

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Abstract

( ) is characterized as an etiological agent of primary atypical pneumonia that specifically infects sheep and goat. In an attempt to better understand the pathogen-host interaction between the invading and airway epithelial cells, we investigated the host inflammatory responses against capsular polysaccharide (designated as CPS) of using sheep bronchial epithelial cells cultured in an air-liquid interface (ALI) model. Results showed that CPS derived from could activate toll-like receptor- (TLR-) mediated inflammatory responses, along with an elevated expression of nuclear factor kappa B (NF- B), activator protein-1 (AP-1), and interferon regulatory factor 3 (IRF3) as well as various inflammatory-associated mediators, representatively including proinflammatory cytokines, such as IL1 , TNF , and IL8, and anti-inflammatory cytokines such as IL10 and TGF of TLR signaling cascade. Mechanistically, the CPS-induced inflammation was TLR initiated and was mediated by activations of both MyD88-dependent and MyD88-independent signaling pathways. Of importance, a blockage of CPS with specific antibody led a significant reduction of -induced inflammatory responses in sheep bronchial epithelial cells. These results suggested that CPS is a key virulent component of , which may play a crucial role in the inflammatory response induced by infections.

Bronchi - microbiology

Epithelial Cells - metabolism

Cytokines - metabolism

Enzyme-Linked Immunosorbent Assay

Signal Transduction

Bacterial Capsules - metabolism

Cells, Cultured

Pneumonia, Mycoplasma - microbiology

Polysaccharides, Bacterial - metabolism

Respiratory System

NF-kappa B - metabolism

Pneumonia, Mycoplasma - veterinary

Transcription Factor AP-1 - metabolism

Host-Pathogen Interactions

Inflammation - metabolism

Animals

Epithelial Cells - microbiology

Sheep

Toll-Like Receptors - metabolism

Mycoplasma ovipneumoniae

Myeloid Differentiation Factor 88 - metabolism

Details

Title: Subtitle: Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells
Creators: Zhongjia Jiang - College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Fuyang Song - College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Yanan Li - College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Di Xue - College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Guangcun Deng - College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Min Li - College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Xiaoming Liu - Institute for Stem Cell Research, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China
Yujiong Wang - College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Resource Type: Journal article
Publication Details: Mediators of inflammation, Vol.2017, pp.9891673-9891673
DOI: 10.1155/2017/9891673
PMID: 28553017
PMCID: PMC5434471
NLM abbreviation: Mediators Inflamm
ISSN: 0962-9351
eISSN: 1466-1861
Publisher: United States
Grant note: DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 31572494, 31460660
Language: English
Date published: 2017
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984025471502771

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