Capsular Polysaccharide of Mycoplasma ovipneumoniae Induces Sheep Airway Epithelial Cell Apoptosis via ROS-Dependent JNK/P38 MAPK Pathways

Zhongjia Jiang; Fuyang Song; Yanan Li; Di Xue; Ning Zhao; Jiamei Zhang; Guangcun Deng; Min Li; Xiaoming Liu; Yujiong Wang

doi:10.1155/2017/6175841

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Capsular Polysaccharide of Mycoplasma ovipneumoniae Induces Sheep Airway Epithelial Cell Apoptosis via ROS-Dependent JNK/P38 MAPK Pathways

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Capsular Polysaccharide of Mycoplasma ovipneumoniae Induces Sheep Airway Epithelial Cell Apoptosis via ROS-Dependent JNK/P38 MAPK Pathways

Zhongjia Jiang, Fuyang Song, Yanan Li, Di Xue, Ning Zhao, Jiamei Zhang, Guangcun Deng, Min Li, Xiaoming Liu and Yujiong Wang

Oxidative medicine and cellular longevity, Vol.2017, 6175841

2017

DOI: 10.1155/2017/6175841

PMCID: PMC5359454

PMID: 28367270

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Abstract

In an attempt to better understand the pathogen-host interaction between invading ( ) and sheep airway epithelial cells, biological effects and possible molecular mechanism of capsular polysaccharide of (CPS) in the induction of cell apoptosis were explored using sheep bronchial epithelial cells cultured in air-liquid interface (ALI). The CPS of was first isolated and purified. Results showed that CPS had a cytotoxic effect by disrupting the integrity of mitochondrial membrane, accompanied with an increase of reactive oxygen species and decrease of mitochondrial membrane potential (ΔΨ ). Of importance, the CPS exhibited an ability to induce caspase-dependent cell apoptosis via both intrinsic and extrinsic apoptotic pathways. Mechanistically, the CPS induced extrinsic cell apoptosis by upregulating FAS/FASL signaling proteins and cleaved-caspase-8 and promoted a ROS-dependent intrinsic cell apoptosis by activating a JNK and p38 signaling but not ERK1/2 signaling of mitogen-activated protein kinases (MAPK) pathways. These findings provide the first evidence that CPS of induces a caspase-dependent apoptosis via both intrinsic and extrinsic apoptotic pathways in sheep bronchial epithelial cells, which may be mainly attributed by a ROS-dependent JNK and p38 MAPK signaling pathways.

Epithelial Cells - metabolism

Reactive Oxygen Species - metabolism

Nitriles - pharmacology

Apoptosis - drug effects

Epithelial Cells - drug effects

Mitogen-Activated Protein Kinase 3 - antagonists & inhibitors

JNK Mitogen-Activated Protein Kinases - metabolism

Membrane Potential, Mitochondrial - drug effects

Caspases - metabolism

p38 Mitogen-Activated Protein Kinases - metabolism

Epithelial Cells - cytology

Bronchi - cytology

Polysaccharides, Bacterial - toxicity

Butadienes - pharmacology

JNK Mitogen-Activated Protein Kinases - antagonists & inhibitors

Mycoplasma ovipneumoniae - metabolism

Mitogen-Activated Protein Kinase 1 - antagonists & inhibitors

Animals

MAP Kinase Signaling System - drug effects

Mitogen-Activated Protein Kinase 3 - metabolism

p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors

Acetylcysteine - pharmacology

Sheep

Protein Kinase Inhibitors - pharmacology

Oxidative Stress - drug effects

Mitogen-Activated Protein Kinase 1 - metabolism

Details

Title: Subtitle: Capsular Polysaccharide of Mycoplasma ovipneumoniae Induces Sheep Airway Epithelial Cell Apoptosis via ROS-Dependent JNK/P38 MAPK Pathways
Creators: Zhongjia Jiang - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Fuyang Song - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Yanan Li - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Di Xue - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Ning Zhao - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China
Jiamei Zhang - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China
Guangcun Deng - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Min Li - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Xiaoming Liu - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China; Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, the General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China
Yujiong Wang - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
Resource Type: Journal article
Publication Details: Oxidative medicine and cellular longevity, Vol.2017, 6175841
DOI: 10.1155/2017/6175841
PMID: 28367270
PMCID: PMC5359454
NLM abbreviation: Oxid Med Cell Longev
ISSN: 1942-0900
eISSN: 1942-0994
Publisher: United States
Grant note: DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 31460660, 31572494
Language: English
Date published: 2017
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984025340602771

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