Journal article
Capture and Stabilization of the Hydroxyl Radical in a Uranyl Peroxide Cluster
Chemistry : a European journal, Vol.29(45), e202300749
08/10/2023
DOI: 10.1002/chem.202300749
PMID: 37249248
Appears in UI Libraries Support Open Access
Abstract
Here we describe the synthesis and characterization of a new uranyl peroxide cluster (UPC), U60Ox30*, which captures and stabilizes oxygen-based free radicals for more than one week. These radical species were first detected with a nitroblue tetrazolium colorimetric assay and U60Ox30* was characterized by single crystal X-ray diffraction as well as infrared (IR), Raman, UV-Vis-NIR, and electron paramagnetic resonance (EPR) spectroscopies. Identification of the free radicals present in U60Ox30* was done via room temperature solid and solution state X-band EPR studies using spin trapping methods. The spin trapping agent 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was definitive for identifying the free radicals in U60Ox30*, which are hydroxyl radicals (•OH) that are stable for up to ten days that also persist upon addition of the metalloenzymes catalase and superoxide dismutase. Addition of the spin trapping agent α-(4-pyridyl N-oxide)-N-tert-butylnitrone (POBN) further confirmed the radicals were oxygen based, and deuteration experiments showed that the origin of the free radicals was from the decomposition of H2O2 in water. These results demonstrate that highly oxidizing species such as the •OH radical can be stabilized in UPCs, which alters our understanding of the role of free radicals present in spent nuclear fuel.
Details
- Title: Subtitle
- Capture and Stabilization of the Hydroxyl Radical in a Uranyl Peroxide Cluster
- Creators
- Brett Lottes - University of IowaKorey P Carter - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Chemistry : a European journal, Vol.29(45), e202300749
- DOI
- 10.1002/chem.202300749
- PMID
- 37249248
- NLM abbreviation
- Chemistry
- eISSN
- 1521-3765
- Publisher
- Wiley
- Grant note
- DOI: 10.13039/100005187, name: U.S. Nuclear Regulatory Commission, award: 31310018M0042
- Language
- English
- Electronic publication date
- 05/30/2023
- Date published
- 08/10/2023
- Academic Unit
- Chemistry
- Record Identifier
- 9984420932902771
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