Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways

Benjamin E Reinking; Elesa W Wedemeyer; Robert M Weiss; Jeffrey L Segar; Thomas D Scholz

doi:10.1186/1475-2840-8-43

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Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways

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Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways

Benjamin E Reinking, Elesa W Wedemeyer, Robert M Weiss, Jeffrey L Segar and Thomas D Scholz

Cardiovascular diabetology, Vol.8(1), pp.43-43

07/31/2009

DOI: 10.1186/1475-2840-8-43

PMCID: PMC2731081

PMID: 19646268

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Abstract

Background: Maternal diabetes affects the developing fetal cardiovascular system. Newborn offspring of diabetic mothers can have a transient cardiomyopathy. We hypothesized that cardiomyopathic remodeling is associated with activation of the mitogen activated protein kinase (MAPK) signaling and apoptotic pathways. Methods: To evaluate the effects of moderate and severe maternal hyperglycemia, pregnant rats were made diabetic with an injection of 50 mg/kg of streptozotocin. Moderately well controlled maternal diabetes was achieved with twice daily glucose checks and insulin injections. No insulin was given to severely diabetic dams. Offspring of moderate and severe diabetic mothers (OMDM and MSDM, respectively) were studied on postnatal days 1 (NB1) and 21 (NB21). Echocardiograms were performed to evaluate left ventricular (LV) dimensions and function. Myocardial MAPK and apoptotic protein levels were measured by Western blot. Results: OMDM had increased cardiac mass at NB1 compared to controls that normalized at NB21. OSDM demonstrated microsomia with relative sparing of cardiac mass and a dilated cardiomyopathy at NB1. In both models, there was a persistent increase in the HW:BW and significant activation of MAPK and apoptotic pathways at NB21. Conclusion: The degree of maternal hyperglycemia determines the type of cardiomyopathy seen in the offspring, while resolution of both the hypertrophic and dilated cardiomyopathies is associated with activation of MAPK signaling and apoptotic pathways.

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Title: Subtitle: Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways
Creators: Benjamin E Reinking - UIHC, Division of Pediatric Cardiology, 200 Hawkins Drive, 2801 JPP, Iowa City, IA 52242-1083, USA
Elesa W Wedemeyer - UIHC, Division of Pediatric Cardiology, 200 Hawkins Drive, 2801 JPP, Iowa City, IA 52242-1083, USA
Robert M Weiss - Departments of Pediatrics and Internal Medicine, University of Iowa, Iowa City, IA USA 52242-1083, USA
Jeffrey L Segar - UIHC, Division of Pediatric Cardiology, 200 Hawkins Drive, 2801 JPP, Iowa City, IA 52242-1083, USA
Thomas D Scholz - UIHC, Division of Pediatric Cardiology, 200 Hawkins Drive, 2801 JPP, Iowa City, IA 52242-1083, USA
Resource Type: Journal article
Publication Details: Cardiovascular diabetology, Vol.8(1), pp.43-43
DOI: 10.1186/1475-2840-8-43
PMID: 19646268
PMCID: PMC2731081
NLM abbreviation: Cardiovasc Diabetol
ISSN: 1475-2840
eISSN: 1475-2840
Publisher: BioMed Central
Language: English
Date published: 07/31/2009
Academic Unit: Cardiology; Stead Family Department of Pediatrics; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Child and Community Health; Internal Medicine
Record Identifier: 9984093373302771

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