Journal article
Cardiovascular Function and Autonomic Regulation in Urethane Anesthetized and Conscious Mice
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.328(1), pp.R133-R144
01/2025
DOI: 10.1152/ajpregu.00097.2024
PMCID: PMC12400817
PMID: 39636660
Abstract
Urethane is widely used for its ability to induce prolonged anesthesia. Variability in previously reported cardiovascular parameters in murine models makes it challenging to definitively evaluate the cardiovascular effects of urethane anesthesia. We aimed to address these challenges, thereby advancing our understanding of urethane's effects on cardiovascular function in mice. In this study, we investigated how urethane anesthesia, with and without supplemental oxygen, impacts heart rate, arterial oxygen saturation (SpO
), blood pressure, and heart rate variability in mice. First, we conducted a literature review and found that data in mice was both limited and lacking in reproduction. Next, we conducted a series of physiological measurements to address gaps in the literature and subjected C57BL/6J mice to three conditions: 1) conscious, 2) urethane-anesthetized, and 3) urethane-anesthetized with supplemental oxygen. Blood pressure, heart rate, SpO
and heart rate variability (via time, frequency, and M-curve analyses) were assessed. We observed an increase in heart rate with urethane anesthesia (p=0.012) compared to the conscious state. Urethane caused a decrease in heart rate variability which was independent of oxygen supplementation. Urethane anesthesia significantly reduced arterial blood pressure (p<0.001) with oxygen-supplemented mice remained hypotensive. Urethane decreased SpO
(p=0.001), which was restored by oxygen supplementation (p<0.001). We did not observe sex effects of urethane anesthesia on blood pressure, heart rate, heart rate variability, or SpO
. Taken together, these results underscore the importance of a cautious approach when using urethane in mice, as urethane significantly impacts arterial blood pressure, heart rate, oxygen saturation, and heart rate variability.
Details
- Title: Subtitle
- Cardiovascular Function and Autonomic Regulation in Urethane Anesthetized and Conscious Mice
- Creators
- Anastasiia Vasileva - University of IowaLaura D M Flores - University of IowaMikhail Vasilyev - University of IowaMichelle A Buckman - University of IowaLara R DeRuisseau - University of Health Sciences and PharmacyMichael H Tomasson - University of IowaMelissa L Bates - University of Iowa
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.328(1), pp.R133-R144
- DOI
- 10.1152/ajpregu.00097.2024
- PMID
- 39636660
- PMCID
- PMC12400817
- NLM abbreviation
- Am J Physiol Regul Integr Comp Physiol
- ISSN
- 0363-6119
- eISSN
- 1522-1490
- Publisher
- American Physiological Society; Rockville
- Grant note
- R21HD099573 / HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) R56HL152365 / HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI)
- Language
- English
- Electronic publication date
- 12/05/2024
- Date published
- 01/2025
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984757062102771
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