Journal article
Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation
EMBO reports, Vol.13(4), pp.331-338
04/2012
DOI: 10.1038/embor.2012.18
PMCID: PMC3321151
PMID: 22370727
Abstract
The efficient formation of a variety of transport vesicles is influenced by the presence of cargo, suggesting that cargo itself might have a defining role in vesicle biogenesis. However, definitive in vivo experiments supporting this concept are lacking, as it is difficult to eliminate endogenous cargo. The Endosomal Sorting Complexes Required for Transport (ESCRT) apparatus sorts ubiquitinated membrane proteins into endosomal intralumenal vesicles (ILVs) that accumulate within multivesicular bodies. Here we show that cargo ubiquitination is required for effective recruitment of the ESCRT machinery onto endosomal membranes and for the subsequent formation of ILVs. © 2012 European Molecular Biology Organization.
Details
- Title: Subtitle
- Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation
- Creators
- Chris MacDonald - University of IowaNicholas J Buchkovich - Cornell UniversityDaniel K Stringer - University of IowaScott D Emr - Cornell UniversityRobert C Piper - University of Iowa
- Resource Type
- Journal article
- Publication Details
- EMBO reports, Vol.13(4), pp.331-338
- DOI
- 10.1038/embor.2012.18
- PMID
- 22370727
- PMCID
- PMC3321151
- NLM abbreviation
- EMBO Rep
- ISSN
- 1469-221X
- eISSN
- 1469-3178
- Publisher
- John Wiley & Sons, Ltd
- Number of pages
- 8
- Language
- English
- Date published
- 04/2012
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297493302771
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