Journal article
Cartilage Abnormalities Associated with Defects of Chondrocytic Primary Cilia in Bardet-Biedl Syndrome Mutant Mice
Journal of orthopaedic research, Vol.27(8), pp.1093-1099
08/2009
DOI: 10.1002/jor.20855
PMCID: PMC3845817
PMID: 19195025
Abstract
Primary cilia are found on nearly every mammalian cell, including osteocytes, fibroblasts, and chondrocytes. However, the functions of primary cilia have not been extensively studied in these cells, particularly chondrocytes. Interestingly, defects in the primary cilium result in skeletal defects such as polydactyly in Bardet-Biedl Syndrome (BBS), a ciliary disorder that also results in obesity, retinopathy, and cognitive impairments (
1
–
4
). Wild-type mice and mutant mice of the ciliary proteins Bbs1, Bbs2, and Bbs6 were evaluated with respect to histological and biochemical differences in chondrocytes from articular cartilage and xiphoid processes. Using immunofluorescence microscopy, chondrocytic cilia were visualized from the load-bearing joints and non-load-bearing xiphoid processes. Significant differences in ciliary morphology were not identified between mutant and wild-type mice. However, after expanding chondrocytes in cell culture and implanting them in solid agarose matrix, it was seen that the fraction of ciliated cells in cultures from mutant mice was significantly lower than in the wild-type cultures (p<.05). In addition, in Safranin-O-stained whole joint sections, Bbs mutant mice had significantly lower articular joint thickness (p<.05) and lower proteoglycan content saturation (p<.05) than wild-type mice. Moreover, there were statistically significant differences of cell distribution between Bbs mutant and wild-type mice (p<.05), indicating that mutant articular cartilage had changes consistent with early signs of osteoarthritis. These data indicate that Bbs genes and their functions in the chondrocytic primary cilium are important for normal articular cartilage maintenance.
Details
- Title: Subtitle
- Cartilage Abnormalities Associated with Defects of Chondrocytic Primary Cilia in Bardet-Biedl Syndrome Mutant Mice
- Creators
- Anjan P Kaushik - Department of Orthopaedic Surgery and Rehabilitation, University of IowaJames A Martin - Department of Orthopaedic Surgery and Rehabilitation, University of IowaQihong Zhang - Department of Pediatrics, University of IowaVal C Sheffield - Department of Pediatrics, University of IowaJose A Morcuende - Department of Orthopaedic Surgery and Rehabilitation, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of orthopaedic research, Vol.27(8), pp.1093-1099
- DOI
- 10.1002/jor.20855
- PMID
- 19195025
- PMCID
- PMC3845817
- ISSN
- 0736-0266
- eISSN
- 1554-527X
- Grant note
- R01 EY011298-11 || EY / National Eye Institute : NEI
- Language
- English
- Date published
- 08/2009
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Medical Genetics and Genomics; Ophthalmology and Visual Sciences
- Record Identifier
- 9984040282202771
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