Cartilage extracellular matrix metabolism differs in serum and synovial fluid

James A MARTIN; Andrew L WILKEY; Richard A BRAND

doi:10.1023/A:1024468101796

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Cartilage extracellular matrix metabolism differs in serum and synovial fluid

Journal article

Peer reviewed

Cartilage extracellular matrix metabolism differs in serum and synovial fluid

James A MARTIN, Andrew L WILKEY and Richard A BRAND

Methods in cell science, Vol.24(4), pp.139-143

2002

DOI: 10.1023/A:1024468101796

PMID: 12843702

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Abstract

Most cartilage explant culture studies assume conventional serum-supplemented growth media are biologically equivalent to the natural synovial fluid which baths cartilage in vivo. Few studies have systematically compared the effects of serum versus synovial fluid in culture. To address this assumption we conducted a series of studies to determine if cartilage matrix synthesis is significantly different in serum-based versus synovial fluid-based media. Normal bovine cartilage explants were cultured in DMEM either alone or supplemented with bovine serum or bovine synovial fluid. Matrix synthesis was measured with radiolabeling techniques. We then compared responses to insulin-like growth factor I (IGF-I, a stimulator of matrix synthesis), and interleukin-1beta (IL-1beta, an inhibitor of matrix synthesis). We observed significantly lower matrix synthesis activity in synovial fluid versus serum. Caution shoud be used in extrapolating studies of cartilage grown in media supplemented with serum rather than synovial fluid.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Details

Title: Subtitle: Cartilage extracellular matrix metabolism differs in serum and synovial fluid
Creators: James A MARTIN - Ponseti Cell and Molecular Biology Laboratory, Department of Orthopaedic Surgery, The University of Iowa, Iowa City, IA 52242, United States
Andrew L WILKEY - Ponseti Cell and Molecular Biology Laboratory, Department of Orthopaedic Surgery, The University of Iowa, Iowa City, IA 52242, United States
Richard A BRAND - Ponseti Cell and Molecular Biology Laboratory, Department of Orthopaedic Surgery, The University of Iowa, Iowa City, IA 52242, United States
Resource Type: Journal article
Publication Details: Methods in cell science, Vol.24(4), pp.139-143
Publisher: Kluwer; Norwell, MA; Dordrecht
DOI: 10.1023/A:1024468101796
PMID: 12843702
ISSN: 1381-5741
eISSN: 1573-0603
Language: English
Date published: 2002
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation
Record Identifier: 9984040589002771

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