Casein kinase II enhances the DNA binding activity of serum response factor

J Robert Manak; Nathalie de Bisschop; Richard M Kris; Ron Prywes

doi:10.1101/gad.4.6.955

Back

Casein kinase II enhances the DNA binding activity of serum response factor

Journal article

Open access

Peer reviewed

Casein kinase II enhances the DNA binding activity of serum response factor

J Robert Manak, Nathalie de Bisschop, Richard M Kris and Ron Prywes

Genes & development, Vol.4(6), pp.955-967

06/1990

DOI: 10.1101/gad.4.6.955

PMID: 2200737

Files and links (1)

url

https://doi.org/10.1101/gad.4.6.955View

Published (Version of record) Open Access

Abstract

Serum response factor (SRF) is a mammalian transcription factor that binds to the serum response element in the enhancer of the c-fos proto-oncogene and thus may mediate serum-induction of c-fos transcription. We report here that the DNA binding activity of recombinant SRF made in Escherichia coli can be greatly enhanced by incubation of the protein with HeLa cell nuclear extract. The enhancing activity is ATP or GTP dependent and cofractionates with a protein kinase that phosphorylates SRF on a specific tryptic peptide. Coincubation with phosphatase blocks the enhancing activity, further suggesting that the enhanced binding activity is due to phosphorylation. The specific tryptic phosphopeptide phosphorylated in vitro is also phosphorylated in vivo, demonstrating that this phosphorylation is physiologically important. We have localized the phosphorylation site by a small deletion mutant. Finally, we show that the kinase activity is provided by casein kinase II (CKII) or a close variant. The potential role of CKII as either a regulatory or constitutive modifier of SRF in vivo will be discussed.

Phosphorylation

Mutation

Protein Kinases - metabolism

Casein Kinases

Humans

Molecular Sequence Data

Nuclear Proteins - metabolism

Guanosine Triphosphate - metabolism

DNA - metabolism

DNA-Binding Proteins - metabolism

Serum Response Factor

Nuclear Proteins - biosynthesis

Escherichia coli - genetics

Adenosine Triphosphate - metabolism

Base Sequence

Cell Extracts

Transcription, Genetic

HeLa Cells

Nuclear Proteins - genetics

Details

Title: Subtitle: Casein kinase II enhances the DNA binding activity of serum response factor
Creators: J Robert Manak - Department of Biological Sciences, Columbia University, New York, New York 10027
Nathalie de Bisschop
Richard M Kris
Ron Prywes
Resource Type: Journal article
Publication Details: Genes & development, Vol.4(6), pp.955-967
DOI: 10.1101/gad.4.6.955
PMID: 2200737
NLM abbreviation: Genes Dev
ISSN: 0890-9369
eISSN: 1549-5477
Publisher: United States
Grant note: CA 50329-01 / NCI NIH HHS
Language: English
Date published: 06/1990
Academic Unit: Stead Family Department of Pediatrics; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9983991944602771

Metrics

35 Record Views

175 Times Cited - Web of Science

See more details