Journal article
Caspase-3 inhibits growth in Saccharomyces cerevisiae without causing cell death
FEBS letters, Vol.446(1), pp.9-14
1999
DOI: 10.1016/S0014-5793(99)00159-3
PMID: 10100604
Abstract
Caspase-3, a member of the caspase family of cell death proteases, cleaves cytoplasmic and nuclear substrates and promotes apoptotic cell death in mammalian cells. Although yeast homologs of apoptotic genes have not been identified, some components of apoptotic pathways retain function in yeast. Here we show that the expression of caspase-3 delays cell growth in
Saccharomyces cerevesiae without causing cell death. Mutation of the caspase-3 QACRG active site abolished effects on yeast growth. Co-expression of caspase inhibitors alleviated growth inhibition in yeast as did the tripeptide caspase inhibitor ZVAD-fmk. These results suggest that substrates for caspase-3 are present in
S. cerevesiae and may participate in the normal cell growth and division processes.
Details
- Title: Subtitle
- Caspase-3 inhibits growth in Saccharomyces cerevisiae without causing cell death
- Creators
- Michael E. Wright - Fred Hutch Cancer CenterDavid K. Han - University of WashingtonLauren Carter - University of WashingtonStanley Fields - Howard Hughes Medical InstituteStephen M. Schwartz - University of WashingtonDavid M. Hockenbery - Fred Hutchinson Cancer Research Center
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.446(1), pp.9-14
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0014-5793(99)00159-3
- PMID
- 10100604
- ISSN
- 0014-5793
- eISSN
- 1873-3468
- Language
- English
- Date published
- 1999
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984297595602771
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