Journal article
Catecholamines increase lung edema clearance in rats with increased left atrial pressure
Journal of applied physiology (1985), Vol.90(3), pp.1088-1094
03/01/2001
DOI: 10.1152/jappl.2001.90.3.1088
PMID: 11181624
Abstract
During hydrostatic pulmonary edema, active Na
+
transport and alveolar fluid reabsorption are decreased. Dopamine (DA) and isoproterenol (ISO) have been shown to increase active Na
+
transport in rat lungs by upregulating Na
+
-K
+
-ATPase in the alveolar epithelium. We studied the effects of DA and ISO in isolated rat lungs with increased left atrial pressure (Pla = 15 cmH
2
O) compared with control rats with normal Pla (Pla = 0). Alveolar fluid reabsorption decreased from control value of 0.51 ± 0.02 to 0.27 ± 0.02 ml/h when Pla was increased to 15 cmH
2
O ( P < 0.001). DA and ISO increased the alveolar fluid reabsorption back to control levels. Treatment with the D
1
antagonist SCH-23390 inhibited the stimulatory effects of DA (0.30 ± 0.02 ml/h), whereas fenoldopam, a specific D
1
-receptor agonist, increased alveolar fluid reabsorption in rats exposed to Pla of 15 cmH
2
O (0.47 ± 0.04 ml/h). Propranolol, a β-adrenergic-receptor antagonist, blocked the stimulatory effects of ISO; however, it did not affect alveolar fluid reabsorption in control or DA-treated rats. Amiloride (a Na
+
channel blocker) and ouabain (a Na
+
-K
+
-ATPase inhibitor), either alone or together, inhibited the stimulatory effects of DA. Colchicine, which disrupts the cellular microtubular transport of ion-transporting proteins to the plasma membrane, inhibited the stimulatory effects of DA, whereas the isomer β-lumicolchicine did not block the stimulatory effects of DA. These data suggest that DA and ISO increase alveolar fluid reabsorption in a model of increased Pla by regulating active Na
+
transport in rat alveolar epithelium. The effects of DA and ISO are mediated by the activation of dopaminergic D
1
receptors and the β-adrenergic receptors, respectively.
Details
- Title: Subtitle
- Catecholamines increase lung edema clearance in rats with increased left atrial pressure
- Creators
- Zaher S. Azzam - Northwestern UniversityFernando J. Saldias - Pontificia Universidad Católica de ChileAlejandro Comellas - Northwestern UniversityKaren M. Ridge - Northwestern UniversityDavid H. Rutschman - Northeastern Illinois UniversityJacob I. Sznajder - Northwestern University
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.90(3), pp.1088-1094
- DOI
- 10.1152/jappl.2001.90.3.1088
- PMID
- 11181624
- ISSN
- 8750-7587
- eISSN
- 1522-1601
- Language
- English
- Date published
- 03/01/2001
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; ICTS; Internal Medicine
- Record Identifier
- 9984359892202771
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